Dilarri Guilherme, Zamuner Caio Felipe Cavicchia, Bacci Mauricio, Ferreira Henrique
Department of General and Applied Biology, São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, SP 13506-900 Brazil.
J Food Sci Technol. 2022 May;59(5):1739-1747. doi: 10.1007/s13197-021-05185-3. Epub 2021 Jun 26.
(. ) is a quarentenary plant pathogen and the causal agent of the citrus canker. . forms biofilms and remains fixed on the surface of plant tissues, especially on leaves and fruits. Considering this, all the citrus fruits have to be sanitized before they can be commercialized. NaOCl is the main sanitizer used to decontaminate fruits in the world. Due to its toxicity, treatment with NaOCl is no longer accepted by some Europe Union countries. Therefore, the aim of this work was to evaluate potassium bicarbonate (KHCO), calcium hydroxide (Ca(OH)), calcium hypochlorite (Ca(OCl)) and peracetic acid (CHCOH) as alternatives to NaOCl for the sanitization of citrus fruit. By monitoring cell respiration and bacterial growth, we determined that peracetic acid and calcium hypochlorite exhibit bactericidal action against . Time-response growth curves and membrane integrity analyses showed that peracetic acid and calcium hypochlorite target the bacterial cytoplasmatic membrane, which is probably responsible for cell death in the first minutes of contact. The simulation of the sanitization process of citrus fruit in packinghouses showed that only peracetic acid exhibited a performance comparable to NaOCl. Among the tested compounds, peracetic acid constitutes an efficient and safer alternative to NaOCl.
(.)是一种四季性植物病原体,也是柑橘溃疡病的致病因子。. 形成生物膜并固定在植物组织表面,尤其是叶片和果实表面。鉴于此,所有柑橘类水果在商业化之前都必须进行消毒处理。次氯酸钠(NaOCl)是世界上用于水果去污的主要消毒剂。由于其毒性,一些欧盟国家已不再接受使用次氯酸钠进行处理。因此,本研究的目的是评估碳酸氢钾(KHCO)、氢氧化钙(Ca(OH))、次氯酸钙(Ca(OCl))和过氧乙酸(CHCOH)作为次氯酸钠的替代品用于柑橘类水果消毒的效果。通过监测细胞呼吸和细菌生长,我们确定过氧乙酸和次氯酸钙对. 具有杀菌作用。时间响应生长曲线和膜完整性分析表明,过氧乙酸和次氯酸钙作用于细菌细胞质膜,这可能是在接触的最初几分钟内导致细胞死亡的原因。对包装厂柑橘类水果消毒过程的模拟表明,只有过氧乙酸表现出与次氯酸钠相当的性能。在所测试的化合物中,过氧乙酸是次氯酸钠的一种高效且更安全的替代品。