Kazybay Bexultan, Sun Qinglei, Dukenbayev Kanat, Nurkesh Ayan Amantaiuly, Xu Na, Kutzhanova Aidana, Razbekova Madina, Kabylda Anar, Yang Qing, Wang Qian, Ma Cuiping, Xie Yingqiu
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Qabanbay Batyr Avenue 53, Nur-Sultan 010000, Kazakhstan.
Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
ACS Omega. 2022 Apr 18;7(17):14465-14477. doi: 10.1021/acsomega.1c03018. eCollection 2022 May 3.
A combination therapy of (RP) with goji () has earned a long history in the prescriptions to promote male health. However, the mechanisms at both molecular and nanoscale quantum levels are unclear. Here, we found that processed RP extract induces apoptosis and cell cycle arrest in cancer cells, thereby inhibiting prostate cancer cell proliferation enhanced by processed goji extract associated with an augment of the nanoscale herbzyme of phosphatase. For network pharmacology analysis, RP-induced PI3K-AKT pathways are essential for both benign prostatic hyperplasia and prostate cancer, and the RP/goji combination induces potent pathways which include androgen and estrogen response, kinase regulation, apoptosis, and prostate cancer singling. In addition, the experimental investigation showed that the prostate cancer cells are sensitive to RP extract for inhibiting colony formation. Finally, the natural compound baicalein found in RP ingredients showed a linked activity of top-ranked signaling targets of kinases including MAPK, AKT, and EGFR by the database of cMAP and HERB. Thus, both the nanozyme and ingredients might contribute to the RP in anti-prostate cancer which can be enhanced by goji extract. The proposed nanoscale RP extract might be of significance in developing novel anti-prostate cancer agents by combining goji compositions and targeted therapy compounds.
淫羊藿（RP）与枸杞（枸杞）的联合疗法在促进男性健康的处方中有着悠久的历史。然而，在分子和纳米级量子水平上的机制尚不清楚。在这里，我们发现加工后的淫羊藿提取物可诱导癌细胞凋亡和细胞周期停滞，从而抑制与磷酸酶纳米级草本酶增加相关的加工枸杞提取物增强的前列腺癌细胞增殖。对于网络药理学分析，淫羊藿诱导的PI3K-AKT途径对良性前列腺增生和前列腺癌都至关重要，淫羊藿/枸杞组合诱导包括雄激素和雌激素反应、激酶调节、细胞凋亡和前列腺癌信号传导在内的有效途径。此外，实验研究表明前列腺癌细胞对淫羊藿提取物抑制集落形成敏感。最后，通过cMAP和HERB数据库，在淫羊藿成分中发现的天然化合物黄芩苷显示出与包括MAPK、AKT和EGFR在内的激酶的顶级信号靶点的关联活性。因此，纳米酶和成分都可能有助于淫羊藿的抗前列腺癌作用，枸杞提取物可以增强这种作用。所提出的纳米级淫羊藿提取物可能在通过结合枸杞成分和靶向治疗化合物开发新型抗前列腺癌药物方面具有重要意义。
