College of Sciences, Northeastern University, Shenyang 110004, China.
ACS Appl Mater Interfaces. 2022 May 18;14(19):22006-22015. doi: 10.1021/acsami.2c05296. Epub 2022 May 9.
Enrichment and separation of specific endogenous molecules are essential for disease diagnosis and the pharmaceutical industry. Although many solid sorbents have been developed for target molecule enrichment, simultaneous separation of multitargets is still a challenge for adsorbents. In this study, we develop a multitarget selective sorbent based on a nanochannel membrane prepared by the anodization of a Ti-Cu alloy. The in situ growth of a metal-organic framework (MOF, herein using Cu-based HKUST-1) in the nanochannels enables the resulting MOF-in-nanochannel membrane to act as a nanofilter. Benefitting from the size-exclusion effect of MOFs and the distinct surface characteristics of each component in the HKUST-1/TiO nanochannels, the as-proposed membranes can be simply operated as a filter and exhibit satisfactory selectivities and enrichment capacities in the separation of aromatic amino acids, histidine-rich proteins, and phosphoproteins. More importantly, the adsorbed multitargets can be further controllably released from the membrane in a sequence via a staged recovery process. The use of this system is envisioned to provide an innovative and potential design for efficient sorption media for the selective enrichment and staged separation of specific biomolecules.
特定内源性分子的富集和分离对于疾病诊断和制药工业至关重要。尽管已经开发出许多用于目标分子富集的固体吸附剂，但同时分离多种靶标仍然是吸附剂面临的挑战。在本研究中，我们开发了一种基于 Ti-Cu 合金阳极氧化制备的纳米通道膜的多靶选择性吸附剂。金属-有机骨架（MOF，本文中使用基于 Cu 的 HKUST-1）在纳米通道中的原位生长使得所得的 MOF-纳米通道膜能够充当纳米滤器。受益于 MOFs 的尺寸排阻效应以及 HKUST-1/TiO 纳米通道中每个组分的独特表面特性，所提出的膜可以简单地用作过滤器，并在芳香族氨基酸、富含组氨酸的蛋白质和磷酸蛋白质的分离中表现出令人满意的选择性和富集能力。更重要的是，吸附的多种靶标可以通过分阶段回收过程从膜中依次可控地释放。预计该系统的使用将为特定生物分子的选择性富集和分阶段分离提供一种高效吸附剂的创新和潜在设计。
