Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City 110004, Liaoning Province, China.
Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City 110004, Liaoning Province, China.
Ecotoxicol Environ Saf. 2022 Jun 15;238:113607. doi: 10.1016/j.ecoenv.2022.113607. Epub 2022 May 6.
Bisphenol A (BPA) is found in many plastics widely used in everyday life and affects the immune system. Previous studies found that the selective G protein coupled estrogen receptor (GPER) agonist G-1 can reduce the inflammation associated with asthma and allergic rhinitis (AR). BPA also interferes with the protective effect of estradiol against myocardial ischemia-reperfusion injury.
We explored whether BPA attenuates the effect of G-1 on inflammation in a mouse AR model.
The AR model was established by sensitizing and stimulating female BALB/c mice with ovalbumin (OVA) and G-1/BPA. Eosinophils, neutrophils, and lymphocyte subsets (including T and B cells) in nasal mucosa and Th2 and Treg cells in the spleen were detected by flow cytometry. Cytokines and transcription factors characteristic of Th2 and Treg cells in nasal mucosa were detected using cytometric bead arrays and quantitative PCR, respectively.
G-1 reduced OVA-induced nasal mucosal inflammation in mice. The proportions of eosinophils, neutrophils, Siglec-F neutrophils, lymphocytes, and T cell subsets were reduced by G-1, and this effect was attenuated by BPA. G-1 significantly decreased the Th2 population and levels of IL-4, IL-5, IL-13 and GATA-3; these effects were attenuated by BPA. The enhanced Treg response (as evidenced by an increased Treg population and higher IL-10 and Foxp3 levels) mediated by G-1 tended to be reduced by BPA.
We found that G-1 reduced OVA-induced nasal mucosal inflammation and significantly decreased the Th2 response, while increasing the Treg response. These effects were attenuated by BPA.
双酚 A（BPA）存在于日常生活中广泛使用的许多塑料中，会影响免疫系统。先前的研究发现，选择性 G 蛋白偶联雌激素受体（GPER）激动剂 G-1 可以减轻与哮喘和过敏性鼻炎（AR）相关的炎症。BPA 还会干扰雌二醇对心肌缺血再灌注损伤的保护作用。
我们探讨了 BPA 是否会减弱 G-1 在 AR 模型中对炎症的作用。
通过卵清蛋白（OVA）和 G-1/BPA 致敏和刺激雌性 BALB/c 小鼠建立 AR 模型。通过流式细胞术检测鼻黏膜中的嗜酸性粒细胞、中性粒细胞和淋巴细胞亚群（包括 T 和 B 细胞）以及脾中的 Th2 和 Treg 细胞。使用细胞因子检测试剂盒和定量 PCR 分别检测鼻黏膜中 Th2 和 Treg 细胞的特征性细胞因子和转录因子。
G-1 减轻了 OVA 诱导的小鼠鼻黏膜炎症。G-1 降低了 Siglec-F 中性粒细胞、淋巴细胞和 T 细胞亚群的比例，这种作用被 BPA 减弱。G-1 显著降低了 Th2 群体和 IL-4、IL-5、IL-13 和 GATA-3 的水平；这些作用被 BPA 减弱。G-1 介导的增强的 Treg 反应（表现为 Treg 群体增加和更高的 IL-10 和 Foxp3 水平）被 BPA 减弱。
我们发现 G-1 减轻了 OVA 诱导的鼻黏膜炎症，显著降低了 Th2 反应，同时增加了 Treg 反应。这些作用被 BPA 减弱。
