Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
J Ethnopharmacol. 2022 Aug 10;294:115349. doi: 10.1016/j.jep.2022.115349. Epub 2022 May 6.
Radix Polygalae, a commonly used traditional Chinese herb, has conventionally functioned in tranquilization and sedation, where anti-inflammation may be the underlying mechanism.
Chronic restraint stress (CRS), a risk factor for the etiology of intestinal disorders, was used in the present study to examine whether Radix Polygalae extract (RPE) could modulate colonic dysfunction in CRS rats.
Wistar rats were exposed to 28-day CRS (6 h daily), and RPE (135 mg/kg and 270 mg/kg) was intragastrically administered 1 h before CRS. Subsequently, the gut microbiota was determined using metagenomic sequencing. Colonic proinflammatory interleukin-1β, -6, and -18 were assayed using qRT-PCR and ELISA. Tight junction proteins were quantified by qRT-PCR and western blotting (WB), and tryptophan metabolic enzymes and metabolites were determined using qRT-PCR and UFLC-QTRAP-5500/MS. Moreover, protein expression of colonic tight junction proteins, NF-κB-NLRP3 signaling involved in the underlying mechanism of RPE were detected by WB.
RPE significantly decreased proinflammatory cytokines and reshaped the gut microbiota, especially the probiotics, including Lactobacillus and Bacteroides. Moreover, RPE could modulate the metabolite contents and enzyme expression associated with colonic tryptophan-kynurenine (TRP-KYN) metabolism and could increase tight junction protein expression in CRS rats. Furthermore, RPE inhibited the activation of NF-κB-NLRP3 signaling in the colon of CRS rats.
RPE could modulate colonic inflammation, colonic microbiota, tight junction, TRP-KYN metabolism and NF-κB-NLRP3 signaling to reach a colonic balance of CRS rats. The present study helped us to better understand and appreciate the various beneficial effects of RPE.
远志，一种常用的传统中药，通常具有镇静和安神的作用，其抗炎作用可能是其潜在的机制。
慢性束缚应激（CRS）是肠道疾病发病的一个危险因素，本研究旨在探讨远志提取物（RPE）是否能调节 CRS 大鼠的结肠功能障碍。
Wistar 大鼠接受 28 天 CRS（每天 6 小时），RPE（135mg/kg 和 270mg/kg）在 CRS 前 1 小时灌胃给药。随后，使用宏基因组测序来确定肠道微生物群。使用 qRT-PCR 和 ELISA 测定结肠促炎细胞因子白细胞介素-1β、-6 和 -18。通过 qRT-PCR 和 Western blot（WB）定量紧密连接蛋白，通过 qRT-PCR 和 UFLC-QTRAP-5500/MS 测定色氨酸代谢酶和代谢物。此外，通过 WB 检测 RPE 作用机制中涉及的结肠紧密连接蛋白、NF-κB-NLRP3 信号的蛋白表达。
RPE 显著降低了促炎细胞因子，并重塑了肠道微生物群，特别是益生菌，包括乳酸杆菌和拟杆菌。此外，RPE 可以调节与结肠色氨酸-犬尿氨酸（TRP-KYN）代谢相关的代谢物含量和酶表达，并增加 CRS 大鼠结肠紧密连接蛋白的表达。此外，RPE 抑制了 CRS 大鼠结肠中 NF-κB-NLRP3 信号的激活。
RPE 可以调节 CRS 大鼠的结肠炎症、结肠微生物群、紧密连接、TRP-KYN 代谢和 NF-κB-NLRP3 信号，以达到结肠的平衡。本研究有助于我们更好地理解和欣赏 RPE 的多种有益作用。
