CooperSugical Fertility Solutions, London, UK.
School of Biosciences, University of Kent, Canterbury, UK.
Hum Fertil (Camb). 2023 Dec;26(5):958-963. doi: 10.1080/14647273.2022.2026498. Epub 2022 May 10.
ICSI is widely recommended for patients undergoing preimplantation genetic testing (PGT), but are sperm a potential source of paternal cell contamination in PGT? Semen samples were obtained from five normozoospermic men consenting to research. From each sample 1, 2, 4, 8 and 10 sperm were collected in PCR tubes and whole genome amplification according to PGT-A and PGT-SR processing protocols was undertaken. None of the 25 samples submitted (a total of 125 sperm) showed evidence of DNA amplification. Thus, paternal cell contamination resulting from using conventional in vitro fertilization (IVF) as the insemination method, carries a low risk of an adverse event or misdiagnosis in PGT-A. Due to the higher risk incurred with PGT-SR, clinics may wish to exercise increased caution and continue using ICSI, while PGT-M involves different processing protocols, presenting a different risk profile.
卵胞浆内单精子注射（ICSI）被广泛推荐用于接受胚胎植入前遗传学检测（PGT）的患者，但精子是否是 PGT 中父系细胞污染的潜在来源？从同意进行研究的五名正常精子症男性中获得精液样本。从每个样本中收集 1、2、4、8 和 10 个精子，并根据 PGT-A 和 PGT-SR 处理方案进行全基因组扩增。提交的 25 个样本（共 125 个精子）均未显示出 DNA 扩增的证据。因此，使用传统的体外受精（IVF）作为授精方法导致的父系细胞污染，在 PGT-A 中发生不良事件或误诊的风险较低。由于 PGT-SR 带来的风险更高，诊所可能希望谨慎行事，继续使用 ICSI，而 PGT-M 涉及不同的处理方案，具有不同的风险特征。
