Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Hitit University, Corum, Turkey.
Department of Medical Histology and Embryology, Hitit University Erol Olcok Training and Research Hospital, Corum, Turkey.
Gynecol Endocrinol. 2022 Jul;38(7):573-576. doi: 10.1080/09513590.2022.2073992. Epub 2022 May 10.
Worldwide, approximately 10-15% of couples is affected by infertility and infertility is associated with disturbances in female and/or male reproductive systems. The aim of the current study is to investigate the relationship between DAT1 (SLC6A3) VNTR polymorphism with female infertility.
Genomic DNA extractions were performed in 98 fertile and 90 infertile females, 3' untranslated region (3' UTR) variable number tandem repeat (VNTR) polymorphism of DAT1/SLC6A3 was determined by the use of Polymerase Chain Reaction (PCR) method.
It has been demonstrated that there was no statistically significant difference between female infertile and fertile groups in the terms of DAT1 genotypes ( > .05). Moreover, there was no significant difference regarding the frequencies of 9R and 10R alleles in infertile and fertile groups ( > .05).
This is the first study for investigating the relationship between DAT1/SLC6A3 gene polymorphism and infertility in females. Our study contributes to the growing awareness of the relationship between dopaminergic system and female infertility despite no significant differences were reported between infertile females and corresponding fertile subjects in DAT1/SLC6A3 gene.
在世界范围内，大约有 10-15%的夫妇受到不孕不育的影响，而不孕不育与女性和/或男性生殖系统的紊乱有关。本研究旨在探讨 DAT1（SLC6A3）VNTR 多态性与女性不孕不育之间的关系。
对 98 名生育能力正常的女性和 90 名不孕不育的女性进行基因组 DNA 提取，采用聚合酶链反应（PCR）法检测 DAT1/SLC6A3 3'非翻译区（3'UTR）可变数串联重复（VNTR）多态性。
研究表明，DAT1 基因型在不孕不育组和生育能力正常组之间没有统计学上的显著差异（ > .05）。此外，不孕不育组和生育能力正常组之间 9R 和 10R 等位基因的频率也没有显著差异（ > .05）。
这是首次研究 DAT1/SLC6A3 基因多态性与女性不孕不育之间的关系。尽管我们的研究表明在 DAT1/SLC6A3 基因中没有报告不孕不育女性和相应生育能力正常女性之间存在显著差异，但我们的研究有助于提高对多巴胺能系统与女性不孕不育之间关系的认识。
