Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Sarmento Leite, 500/305, Porto Alegre, RS, 90050-170, Brazil.
Neurochem Res. 2022 Aug;47(8):2307-2316. doi: 10.1007/s11064-022-03618-9. Epub 2022 May 10.
Zebrafish larvae have been widely used in neuroscience and drug research and development. In the larval stage, zebrafish present a broad behavioral repertoire and physiological responses similar to adults. Curcumin (CUR), a major component of Curcuma longa L. (Zingiberaceae), has demonstrated the ability to modulate several neurobiological processes relevant to mental disorders in animal models. However, the low bioavailability of this compound can compromise its in vivo biological potential. Interestingly, it has been shown that micronization can increase the biological effects of several compounds. Thus, in this study, we compared the effects of acute exposure for 30 min to the following solutions: water (control), 0.1% DMSO (vehicle), 1 μM CUR, or 1 μM micronized curcumin (MC) in zebrafish larvae 7 days post-fertilization (dpf). We analyzed locomotor activity (open tank test), anxiety (light/dark test), and avoidance behavior (aversive stimulus test). Moreover, we evaluated parameters of oxidative status (thiobarbituric acid reactive substances and non-protein thiols levels). MC increased the total distance traveled and absolute turn angle in the open tank test. There were no significant differences in the other behavioral or neurochemical outcomes. The increase in locomotion induced by MC may be associated with a stimulant effect on the central nervous system, which was evidenced by the micronization process.
斑马鱼幼鱼在神经科学和药物研发中被广泛应用。在幼鱼阶段，斑马鱼表现出广泛的行为表现和与成年动物相似的生理反应。姜黄素（CUR）是姜黄（Zingiberaceae）的主要成分之一，已证明其能够调节动物模型中与精神障碍相关的几种神经生物学过程。然而，该化合物的生物利用度低可能会影响其体内的生物学潜力。有趣的是，已经表明微粉化可以增加几种化合物的生物学效应。因此，在这项研究中，我们比较了以下溶液对受精后 7 天的斑马鱼幼鱼 30 分钟急性暴露的影响：水（对照）、0.1% DMSO（载体）、1 μM CUR 或 1 μM 微粉化姜黄素（MC）。我们分析了运动活性（开放箱测试）、焦虑（明暗测试）和回避行为（厌恶刺激测试）。此外，我们评估了氧化状态的参数（硫代巴比妥酸反应物质和非蛋白巯基水平）。MC 增加了开放箱测试中的总行进距离和绝对转弯角度。其他行为或神经化学结果没有显著差异。MC 诱导的运动增加可能与中枢神经系统的刺激作用有关，这可以通过微粉化过程得到证明。
