Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands.
ACS Sens. 2022 May 27;7(5):1467-1475. doi: 10.1021/acssensors.2c00215. Epub 2022 May 10.
This work presents an imprinted polymer-based thermal biomimetic sensor for the detection of . A novel and facile bacteria imprinting protocol for polydimethylsiloxane (PDMS) films was investigated, and these receptor layers were functionalized with graphene oxide (GO) in order to improve the overall sensitivity of the sensor. Upon the recognition and binding of the target to the densely imprinted polymers, a concentration-dependent measurable change in temperature was observed. The limit of detection attained for the sensor employing PDMS-GO imprints was 80 ± 10 CFU/mL, a full order lower than neat PDMS imprints (670 ± 140 CFU/mL), illustrating the beneficial effect of the dopant on the thermo-dynamical properties of the interfacial layer. A parallel benchmarking of the thermal sensor with a commercial impedance analyzer was performed in order to prove the possibility of using the developed PDMS-GO receptors with multiple readout platforms. Moreover, , and an additional strain were employed as analogue species for the assessment of the selectivity of the device. Finally, because of the potential that this biomimetic platform possesses as a low-cost, rapid, and on-site tool for monitoring contamination in food safety applications, spiked fruit juice was analyzed as a real sample. Reproducible and sensitive results fulfill the limit requirements of the applicable European microbiological regulation.
本工作提出了一种基于印迹聚合物的热仿生传感器，用于检测 。研究了一种新颖且简便的用于聚二甲基硅氧烷（PDMS）薄膜的细菌印迹协议，并且这些受体层用氧化石墨烯（GO）进行了功能化，以提高传感器的整体灵敏度。在目标与密集印迹聚合物的识别和结合后，观察到与浓度相关的可测量的温度变化。采用 PDMS-GO 印迹的传感器的检测限达到 80 ± 10 CFU/mL，比纯 PDMS 印迹（670 ± 140 CFU/mL）低整整一个数量级，这说明了掺杂剂对界面层热力学性质的有益影响。为了证明开发的 PDMS-GO 受体与多种读出平台兼容的可能性，对热传感器进行了与商业阻抗分析仪的平行基准测试。此外，还使用 、 和另外 菌株作为模拟物来评估该设备的选择性。最后，由于这种仿生平台具有作为低成本、快速和现场工具用于食品安全应用中监测 污染的潜力，因此分析了加标果汁作为实际样品。可重复且灵敏的结果满足适用的欧洲微生物法规的要求。
