Mamede A P, Marques M P M, Vassalo A R, Cunha E, Gonçalves D, Parker S F, Kockelmann W, Batista de Carvalho L A E
Molecular Physical Chemistry R&D Unit, Department of Chemistry, University of Coimbra 3004-535 Coimbra Portugal
Department of Life Sciences, University of Coimbra 3004-535 Coimbra Portugal.
RSC Adv. 2019 Nov 11;9(63):36640-36648. doi: 10.1039/c9ra07728f.
The first neutron diffraction study of human burned bone is reported, aiming at a comprehensive elucidation of the heat-induced bone diagenesis process. Chemical and crystallinity changes were probed in different types of bone (femur, humerus and tibia) upon heating to different maximum temperatures (from 400 to 1000 °C, under aerobic conditions). Fourier transform infrared spectroscopy has provided valuable complementary information. Noticeable crystallographic and domain size variations were detected, mainly between 700 and 900 °C, the high temperature interval (>700 °C) corresponding to an organized, highly symmetric inorganic bone matrix, virtually devoid of carbonates and organic constituents, while the lower range (<700 °C) revealed a considerably lower crystallinity associated with the presence of carbonates, lipids and collagen. This work contributes to a better understanding of heat-induced changes in bone and is therefore relevant for archaeology, biomaterials and forensic science.
本文报道了对人类烧焦骨骼的首次中子衍射研究，旨在全面阐明热诱导骨成岩作用过程。在有氧条件下，将不同类型的骨骼（股骨、肱骨和胫骨）加热到不同的最高温度（400至1000°C），探测其化学和结晶度变化。傅里叶变换红外光谱提供了有价值的补充信息。检测到明显的晶体学和畴尺寸变化，主要在700至900°C之间，高温区间（>700°C）对应于一种有组织、高度对称的无机骨基质，几乎不含碳酸盐和有机成分，而较低温度范围（<700°C）显示出与碳酸盐、脂质和胶原蛋白的存在相关的较低结晶度。这项工作有助于更好地理解热诱导的骨骼变化，因此对考古学、生物材料和法医学具有重要意义。
