Silver nanoparticles undergo oxidative dissolution in water upon storage. This occurs in pure water as well as in more complex media, including natural environments, biological tissues, and cell culture media. However, the dissolution leads to the reprecipitation of silver chloride as chloride is present in almost all relevant environments. The discrimination between dissolved silver species (ions and silver complexes) and dispersed (solid) species does not take this into account because all solid species (metallic silver and silver chloride) are isolated together. By applying a chemical separation procedure, we show that it is possible to quantify silver, silver chloride, and dissolved silver species after immersion into a typical cell culture medium (DMEM + 10% FCS). During the dissolution of metallic silver nanoparticles, about half of the dissolved silver is reprecipitated as solid silver chloride, the mere analysis of the soluble silver species does not reflect the true situation. The separation protocol is suitable for all chloride-containing media in the presence or in the absence of biomolecules.