Antsiferova Anna A, Kopaeva Marina Yu, Kochkin Vyacheslav N, Kashkarov Pavel K
National Research Center "Kurchatov Institute", 1, Akademika Kurchatova sq., 123182 Moscow, Russia.
Moscow Institute of Physics and Technologies, 9, Institutskii Lane, 141700 Dolgoprudny, Moscow Region, Russia.
Nanomaterials (Basel). 2021 Nov 26;11(12):3204. doi: 10.3390/nano11123204.
Since ancient times, silver has been known for its pronounced bactericidal, antiviral and fungicidal properties. Currently, nanoparticles of this metal are widely used in the food, light and pharmaceutical industries, as well as in medicine. Silver in any form can have a toxic effect not only on pathogens, but also on healthy cells. The biological activity and bioavailability of silver preparations depend on the degree of their solubility in water. In addition, the maximum permissible concentration of soluble forms of silver is an order of magnitude lower than that of insoluble forms. This makes nanoparticles of silver with a hydrophilic coating that form stable colloidal solutions in aqueous media potentially unsafe objects. In this work, we studied the kinetics of the accumulation of silver nanoparticles with an average size of 34 ± 5 nm stabilized with polyvinylpyrrolidone in the organs of laboratory C57Bl/6 mice. The administration of nanoparticles was carried out orally for 30, 60, 120 and 180 days at the dose of 50 µg/day/animal. All the mice developed and gained weight normally during the experiment. No adverse effects were observed. Determination of the silver content in biological tissues of mammals was accomplished by neutron activation analysis. The masses and concentrations of silver in the brain and its different sections (hippocampus, cerebellum, cortex and remnants), as well as in the lungs, testes, liver, blood, kidneys, spleen and heart, were determined. The injection times at which the accumulation curves reached saturation were established. An extremely high accumulation of silver in the testes was shown at 120 days of administration, and a significant accumulation of silver in the lungs and brain was observed. The accumulation of silver in all parts of the brain except the cortex was significant, and its trend was similar to that in the whole brain.
自古以来，银就因其显著的杀菌、抗病毒和杀真菌特性而闻名。目前，这种金属的纳米颗粒广泛应用于食品、轻工业和制药行业以及医学领域。任何形式的银不仅会对病原体产生毒性作用，还会对健康细胞产生毒性作用。银制剂的生物活性和生物利用度取决于其在水中的溶解度。此外，可溶性银的最大允许浓度比不溶性银低一个数量级。这使得具有亲水性涂层且能在水性介质中形成稳定胶体溶液的银纳米颗粒成为潜在的不安全物质。在这项工作中，我们研究了用聚乙烯吡咯烷酮稳定的平均尺寸为34±5纳米的银纳米颗粒在实验室C57Bl/6小鼠器官中的积累动力学。以50微克/天/动物的剂量对纳米颗粒进行为期30、60、120和180天的口服给药。在实验过程中，所有小鼠生长发育正常且体重增加。未观察到不良反应。通过中子活化分析测定哺乳动物生物组织中的银含量。测定了大脑及其不同部分（海马体、小脑、皮质和残余部分）以及肺、睾丸、肝脏、血液、肾脏、脾脏和心脏中的银质量和浓度。确定了积累曲线达到饱和的注射时间。给药120天时，睾丸中显示出极高的银积累，并且在肺和大脑中观察到银的显著积累。除皮质外，大脑所有部分的银积累都很显著，其趋势与整个大脑相似。
