Lai Chenhuan, Tu Maobing, Yong Qiang, Yu Shiyuan
Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University 159 Longpan Road Nanjing Jiangsu 210037 China.
Forest Products Laboratory and Center for Bioenergy and Bioproducts, Auburn University Auburn Alabama 36849 USA.
RSC Adv. 2018 Apr 12;8(25):13835-13841. doi: 10.1039/c8ra00902c. eCollection 2018 Apr 11.
The effect of ethanol organosolv lignin (EOL) on enzymatic hydrolysis was examined at pH 4.8-6.0. The addition of EOL prepared from sweetgum enhanced the enzymatic hydrolysis of organosolv-pretreated loblolly pine (OPLP) by 38.8% and 88.0% at pH 4.8 and 5.6, respectively. The addition of EOL prepared from loblolly pine inhibited the enzymatic hydrolysis of OPLP at pH 4.8 but improved it by 43.0% at pH 5.6. This suggests that the addition of EOL and increase in pH act synergistically to improve the enzymatic hydrolysis of OPLP. The effect of EOL addition on cellulase adsorption onto residual lignins was examined. The results revealed that increasing the pH intensified the suppression of non-productive binding between enzymes and residual lignins by EOL. The potential stabilization effects of EOL on enzymes can contribute to the improvement of enzymatic hydrolysis with EOL at higher pH.
在pH值4.8 - 6.0条件下考察了乙醇有机溶剂木质素（EOL）对酶水解的影响。添加由胶皮枫香树制备的EOL，在pH值4.8和5.6时，分别使有机溶剂预处理火炬松（OPLP）的酶水解提高了38.8%和88.0%。添加由火炬松制备的EOL，在pH值4.8时抑制了OPLP的酶水解，但在pH值5.6时使其提高了43.0%。这表明添加EOL和提高pH值协同作用可改善OPLP的酶水解。考察了添加EOL对纤维素酶吸附到残余木质素上的影响。结果表明，提高pH值增强了EOL对酶与残余木质素之间非生产性结合的抑制作用。EOL对酶的潜在稳定作用有助于在较高pH值下用EOL改善酶水解。
