Sar M, Culler M D, McGimsey W C, Negro-Vilar A
Neuroendocrinology. 1987 Feb;45(2):172-5. doi: 10.1159/000124720.
Using specific rabbit anti-GAP (gonadotropin-releasing hormone-associated peptide) serum, we have immunocytochemically localized GAP in the rat brain. Immunostaining of neuronal perikarya, fibers and terminals was demonstrated with GAP antiserum under conditions of tissue preparation which make immunostaining with LHRH antisera difficult or undetectable. GAP-immunoreactive perikarya were observed in sections of perfused or nonperfused brains without colchicine pretreatment. Using a double immunoperoxidase staining method, both GAP and LHRH immunoreactivities were shown to coexist in the same neurons. The common distribution of LHRH and GAP immunoreactivity in the rat brain is strongly supportive of GAP representing the non-LHRH portion of the LHRH precursor. The use of GAP antisera that can distinguish between LHRH and the remaining portion of its prohormone represents a valuable tool for studies of LHRH-prohormone processing and distribution.
我们使用特异性兔抗促性腺激素释放激素相关肽（GAP）血清，通过免疫细胞化学方法在大鼠脑中定位了GAP。在那些使促黄体生成素释放激素（LHRH）抗血清难以进行免疫染色或无法检测到免疫染色的组织制备条件下，GAP抗血清显示出对神经元胞体、纤维和终末的免疫染色。在未经秋水仙碱预处理的灌注或未灌注脑切片中观察到了GAP免疫反应性胞体。使用双重免疫过氧化物酶染色方法，发现GAP和LHRH免疫反应性共存于同一神经元中。LHRH和GAP免疫反应性在大鼠脑中的共同分布有力地支持了GAP代表LHRH前体中非LHRH部分的观点。能够区分LHRH及其激素原其余部分的GAP抗血清的使用，是研究LHRH激素原加工和分布的一种有价值的工具。
