Subramanyam Reddy Chinreddy, Kaul Tanushri, Abdel Motelb Khaled Fathy, Sony Sonia Khan, Bharti Jyotsna, Verma Rachana
Department of Botany, CSSR and SRRM Degree College, Kamalapuram, Kadapa District, Andhra Pradesh-516219, India.
Nutritional Improvement of Crops Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.
Bioinformation. 2021 Aug 31;17(8):727-730. doi: 10.6026/97320630017727. eCollection 2021.
Purple acid phosphatases belong to metallo-phosphatase family. Intracellular phosphatases are crucial for phosphorus (P) distribution in the cell and are highly induced in phosphorus-deprived conditions in the soil. Disparate PAP isoforms exist within discrete subcellular compartments in Setaria italica and their expression in P deprived conditions fosters phosphorus amelioration. We isolated the SiPAP18 gene and developed the homology SiPAP18 protein model based on the crystal structure of the Kidney bean PvPAP (PDB ID: 2QFP) as template (sequence similarity 42.7%) using Modeller 9.12 with adequate validation. Structure model analysis shows the significance of five conserved signatures with seven metal-paired amino acid residues during P-deprivation induced phosphorus amelioration.
紫色酸性磷酸酶属于金属磷酸酶家族。细胞内磷酸酶对于细胞内磷（P）的分布至关重要，并且在土壤缺磷条件下会被高度诱导。在谷子的不同亚细胞区室中存在不同的PAP亚型，它们在缺磷条件下的表达促进了磷的改善。我们分离了SiPAP18基因，并以菜豆PvPAP的晶体结构（PDB ID：2QFP）为模板，使用Modeller 9.12并经过充分验证，构建了同源SiPAP18蛋白模型（序列相似性42.7%）。结构模型分析显示了五个保守特征以及七个金属配对氨基酸残基在缺磷诱导的磷改善过程中的重要性。
