Chen Hsiao-Chien, Cheng Chung-Yi, Chen Li-You, Chang Chun-Chao, Yang Chih-Ping, Mai Fu-Der, Liao Wen-Chieh, Chang Hung-Ming, Liu Yu-Chuan
Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University 250 Wuxing St. Taipei 11031 Taiwan
Division of Nephrology, Department of Internal Medicine, Taipei Medical University Wan Fang Hospital 111 Hsing-Long Rd., Sec. 3 Taipei 116 Taiwan.
RSC Adv. 2018 Mar 5;8(18):9618-9626. doi: 10.1039/c7ra13559a.
The role of the hepato-protective agent plasmon-activated water (PAW) as an innovative anti-oxidant during chronic sleep deprivation (SD) is realized in this study. PAW possesses reduced hydrogen-bonded structure, higher chemical potential and significant anti-oxidative properties. tests using rat liver cell line (Clone-9) have demonstrated that PAW is non-cytotoxic and does not change the cellular migration capacity. The experiment on SD rats suffering from intense oxidative damage to the liver, an extremely common phenomenon in the present-time with deleterious effects on metabolic function, is performed by feeding PAW to replace deionized (DI) water. Experimental results indicate that PAW markedly reduces oxidative stress with enhanced bioenergetics in hepatocytes. PAW also effectively restores hepatocytic -membrane ion homeostasis, preserves membranous structures, and successfully improves liver function and metabolic activity. In addition, the hepato-protective effects of PAW are evidently demonstrated by the reduced values of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) and the recovery of total protein and albumin levels. With clear evidences of PAW for protecting liver from SD-induced injury, delivering PAW as a powerful hepato-protective agent should be worthy of trailblazing new clinical trials in a healthier, more natural, and more convenient way.
本研究揭示了肝保护剂等离子体激活水（PAW）在慢性睡眠剥夺（SD）期间作为一种创新抗氧化剂的作用。PAW具有减少的氢键结构、更高的化学势和显著的抗氧化性能。使用大鼠肝细胞系（Clone-9）进行的测试表明，PAW无细胞毒性且不改变细胞迁移能力。通过喂食PAW替代去离子水（DI），对患有肝脏强烈氧化损伤的SD大鼠进行实验，肝脏氧化损伤在当下是一种极其常见的现象，对代谢功能具有有害影响。实验结果表明，PAW显著降低氧化应激，增强肝细胞的生物能量学。PAW还能有效恢复肝细胞 - 膜离子稳态，保留膜结构，并成功改善肝功能和代谢活性。此外，PAW的肝保护作用通过谷草转氨酶（GOT）和谷丙转氨酶（GPT）值的降低以及总蛋白和白蛋白水平的恢复得到明显证明。有明确证据表明PAW可保护肝脏免受SD诱导的损伤，以更健康、更天然、更便捷的方式将PAW作为一种强大的肝保护剂进行开创性的新临床试验应该是值得的。
