Liu Yang, Li Xiaoming, Zhao Jianwei, Wang Dongbo, Yang Qi, Zeng Guangming
College of Environmental Science and Engineering, Hunan University Changsha 410082 P. R. China
Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education Changsha 410082 P. R. China.
RSC Adv. 2018 Jan 16;8(6):3321-3327. doi: 10.1039/c7ra12886j. eCollection 2018 Jan 12.
Carbon sources are essential for biological phosphorus removal (BPR); the carbon sources, however, are often inadequate in municipal wastewater treatment plants. This study demonstrated the feasibility of sludge fermentation liquid enhanced by biosurfactant alkylpolyglycosides (APG) as carbon sources to improve the performance of BPR in the novel oxic/extended idle (O/EI) reactor and the underlying mechanism was also investigated. The results showed that APG induced fermentation liquid could enhance the BPR performance in the O/EI reactor, and the BPR efficiency was 95.2%, which was significantly higher than that in the conventional anaerobic/oxic (A/O) reactor. Mechanism investigation showed that compared with the A/O reactor, the O/EI reactor enriched more polyphosphate accumulating organisms (PAOs) (38.2%), but less glycogen accumulating organisms (GAOs) when the APG-induced fermentation liquid was used as carbon source. The transformations of the polyhydroxyalkanoates (PHA) and glycogen in the O/EI reactor were lower than those in the A/O reactor. Further study found that the activities of polyphosphate kinase (PPK) and acetyl-CoA synthases (ACS) in the O/EI reactor were significantly higher than those of the A/O reactor, which was consistent with the higher BPR efficiency in the O/EI reactor.
碳源对于生物除磷（BPR）至关重要；然而，在城市污水处理厂中碳源往往不足。本研究证明了用生物表面活性剂烷基多苷（APG）强化的污泥发酵液作为碳源来提高新型好氧/延长闲置（O/EI）反应器中BPR性能的可行性，并对其潜在机制进行了研究。结果表明，APG诱导的发酵液可提高O/EI反应器中的BPR性能，BPR效率为95.2%，显著高于传统厌氧/好氧（A/O）反应器。机制研究表明，与A/O反应器相比，当使用APG诱导的发酵液作为碳源时，O/EI反应器中富集了更多的聚磷菌（PAOs）（38.2%），但糖原积累菌（GAOs）较少。O/EI反应器中聚羟基脂肪酸酯（PHA）和糖原的转化低于A/O反应器。进一步研究发现，O/EI反应器中聚磷酸激酶（PPK）和乙酰辅酶A合成酶（ACS）的活性显著高于A/O反应器，这与O/EI反应器中较高的BPR效率一致。
