Zhang Wenjuan, Yang Hanxiao, Li Nan, Zhao Na
Key Laboratory of Macromolecular Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry & Chemical Engineering, Shaanxi Normal University Xi'an 710119 China
RSC Adv. 2018 Apr 20;8(27):14995-15000. doi: 10.1039/c8ra01786g. eCollection 2018 Apr 18.
In this work, we report a fluorescent probe (TPEQN-P) for detecting alkaline phosphatase (ALP) with high sensitivity and monitoring its activity based on the specific aggregation-induced emission (AIE) effect. TPEQN-P can be constructed by conjugating the phosphate moiety into an AIE molecule (TPE-QI) through a reactive -hydroxybenzyl group, which exhibits very weak emission in aqueous media due to its good water solubility. In the presence of ALP, TPEQN-P undergoes dephosphorylation and releases the hydrolysis product TPE-QI, which is intensely fluorescent because of its poor water solubility. The detection limit for ALP using TPEQN-P can be as low as 0.0077 U L with a linear range of 0-30 mU mL in solution. TPEQN-P also shows excellent applicability in serum samples, demonstrating potential applications in clinical diagnosis and biomedical research. TPEQN-P also can be applied for the determination of ALP activity and in ALP inhibitor screening.
在本工作中，我们报道了一种荧光探针（TPEQN-P），它基于特定的聚集诱导发光（AIE）效应，能够高灵敏度地检测碱性磷酸酶（ALP）并监测其活性。TPEQN-P可通过将磷酸基团通过活性羟苄基连接到AIE分子（TPE-QI）上构建而成，由于其良好的水溶性，TPEQN-P在水性介质中发射非常微弱的光。在碱性磷酸酶存在的情况下，TPEQN-P发生去磷酸化反应并释放出水解产物TPE-QI，由于其水溶性差，TPE-QI具有强烈的荧光。使用TPEQN-P检测碱性磷酸酶的检测限低至0.0077 U/L，在溶液中的线性范围为0 - 30 mU/mL。TPEQN-P在血清样本中也显示出优异的适用性，证明了其在临床诊断和生物医学研究中的潜在应用价值。TPEQN-P还可用于碱性磷酸酶活性的测定以及碱性磷酸酶抑制剂的筛选。
