The cotreatment of landfill leachate and high-nitrate wastewater using SBRs: evaluation of denitrification performance and microbial analysis.

Resourceful disposal of landfill leachate has always been an intractable worldwide problem. This study was conducted to investigate the feasibility of biologically treating a combined waste stream of landfill leachate and high-concentration nitrate nitrogen (high-nitrate) wastewater. Raw landfill leachate was pretreated using anaerobic fermentation and ammonia stripping to improve biodegradability. The control sequencing batch reactor (SBR, named R0) was fed only with synthetic high-nitrate wastewater with sodium acetate as the carbon source, whereas the other experimental SBR (named R1) was loaded with mixtures containing leachates. Excessive increase in leachate adversely affected the cotreatment, and it was concluded that the landfill leachate volume ratio should never exceed 7.5% of the total wastewater (14% of the initial COD) based on further batch experiments. The maximum specific denitrification rate of 58.05 mg NO -N (gVSS h) was attained in R1, while that of 32.32 mg NO -N (gVSS h) was obtained in R0. Illumina MiSeq sequencing revealed that adding landfill leachate did not change the fact that , , and dominant in the sodium acetate supported the denitrification systems, but led to the adjustment of their relative abundance. Moreover, the G, K, S, and B denitrifying genes exhibited increased abundance by 138-980% in the cotreated system, which was confirmed by q-PCR analyses. These findings reveal that the denitrification efficiency of activated sludge in SBR cotreated with landfill leachate and high-nitrate wastewater significantly improved, and this may contribute toward the understanding of the molecular mechanisms of biological denitrification under the blending treatment of leachate and high-nitrate wastewater.