Xu Wei, Dai Qingzhou, Wang Yuzhi, Hu Xiaojian, Xu Panli, Ni Rui, Meng Jiaojiao
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University Changsha 410082 P. R. China
Department of Chemistry, School of Basic Medicine, Changsha Medical University Changsha 410219 P. R. China.
RSC Adv. 2018 Jun 13;8(39):21850-21856. doi: 10.1039/c8ra03818j.
A novel magnetic (FeO) surface molecularly imprinted polymer (MIP) based on ionic liquid (IL) (FeO@VTEO@IL-MIPs) was prepared for the selective extraction of lysozyme (Lys). As the functional monomer of the MIPs, an imidazolium-based IL with vinyl groups was prepared. It can provide multiple interactions with template molecules. The amount of IL was optimized (200 mg). Fourier transform infrared spectrometry (FT-IR), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA) and a vibrating sample magnetometer (VSM) were used to characterize the MIP. The results indicate the successful formation of an imprinting polymer layer. The concentration of Lys in the supernatant was determined by UV-vis spectrophotometry at a wavelength of 280 nm. The maximum adsorption capability of the MIP is 213.7 mg g and the imprinting factor (IF) is 2.02. It took 2.5 h for the MIP to attain adsorption equilibrium. The structure of the protein was evaluated using circular dichroism (CD) spectra and UV-visible spectra. The adsorption performance was further investigated in detail by selective adsorption experiments, competitive rebinding tests, and reusability and stability experiments. Furthermore, it was utilized to separate the template protein from a mixture of proteins and real samples successfully because of the high adsorption capacity for Lys.
制备了一种基于离子液体（IL）的新型磁性（FeO）表面分子印迹聚合物（MIP）（FeO@VTEO@IL-MIPs），用于选择性萃取溶菌酶（Lys）。作为MIPs的功能单体，制备了一种含乙烯基的咪唑基离子液体。它可以与模板分子提供多种相互作用。优化了离子液体的用量（200mg）。采用傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、动态光散射（DLS）、热重分析（TGA）和振动样品磁强计（VSM）对MIP进行表征。结果表明成功形成了印迹聚合物层。通过紫外可见分光光度法在280nm波长下测定上清液中Lys的浓度。MIP的最大吸附容量为213.7mg/g，印迹因子（IF）为2.02。MIP达到吸附平衡需要2.5小时。使用圆二色性（CD）光谱和紫外可见光谱评估蛋白质的结构。通过选择性吸附实验、竞争性再结合试验以及再利用性和稳定性实验进一步详细研究了吸附性能。此外，由于对Lys具有高吸附容量，它成功地用于从蛋白质混合物和实际样品中分离模板蛋白质。
