Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, 8165131378, Iran.
Mikrochim Acta. 2018 Mar 27;185(4):241. doi: 10.1007/s00604-018-2745-2.
The authors introduce a new kind of surface artificial biomimetic receptor, referred to as aptameric imprinted polymer (AIP), for separation of biological macromolecules. Highly dispersed magnetic nanoparticles (MNPs) were coated with silica and then functionalized with methacrylate groups via silane chemistry. The aptamer was covalently immobilized on the surface of nanoparticles via a "thiol-ene" click reaction. Once the target analyte (bovine serum albumin; BSA) has bound to the aptamer, a polymer is created by 2-dimensional copolymerization of short-length poly(ethylene glycol) and (3-aminopropyl)triethoxysilane. Following removal of BSA from the polymer, the AIP-MNPs presented here can selectively capture BSA with a specific absorbance (κ) as high as 65. When using this AIP, the recovery of BSA from spiked real biological samples is >97%, and the adsorption capacity is as high as 146 mg g. In our perception, this method has a wide scope in that it may be applied to the specific extraction of numerous other biomolecules. Graphical abstract Schematic presentation of the AIP (aptamer-imprinted polymer) introduced here. The surface of silica coated magnetic nanoparticles is modified with a polymer that is covalently modified with an aptamer against bovine serum albumin (BSA).
作者介绍了一种新型的表面人工仿生受体，称为适体印迹聚合物（AIP），用于分离生物大分子。高度分散的磁性纳米粒子（MNPs）通过硅烷化学被涂覆有硅烷和甲基丙烯酰基。适体通过“硫醇-烯”点击反应共价固定在纳米粒子表面上。一旦目标分析物（牛血清白蛋白；BSA）与适体结合，就会通过短长度聚（乙二醇）和（3-氨丙基）三乙氧基硅烷的 2 维共聚来生成聚合物。从聚合物中除去 BSA 后，本文提出的 AIP-MNPs 可以用高达 65 的特定吸收率（κ）选择性地捕获 BSA。当使用这种 AIP 时，从加标实际生物样品中回收 BSA 的回收率>97%，并且吸附容量高达 146mg g。在我们看来，这种方法具有广泛的应用范围，因为它可能适用于许多其他生物分子的特异性提取。 示意图介绍了这里介绍的 AIP（适体印迹聚合物）。在涂覆有硅烷的磁性纳米粒子的表面上修饰有与牛血清白蛋白（BSA）的聚合物共价修饰的聚合物。
