Maryška Michal, Fojtíková Lucie, Jurok Radek, Holubová Barbora, Lapčík Oldřich, Kuchař Martin
Department of Chemistry of Natural Compounds, University of Chemistry and Technology Technická 5, 166 28 Praha 6 - Dejvice Czech Republic
Forensic Laboratory of Biologically Active Substances, University of Chemistry and Technology Technická 3, 166 28 Praha 6 - Dejvice Czech Republic.
RSC Adv. 2018 May 1;8(29):16243-16250. doi: 10.1039/c8ra02528b. eCollection 2018 Apr 27.
Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Therefore we synthesized novel haptens derived from tryptamine-based drugs, with ,-dimethyltryptamine (DMT), 5-methoxy-,-dimethyltryptamine (5-MeO-DMT) and ,-diisopropyltryptamine (DiPT) selected as the target analytes. Their structures were modified with a short linker ended with a carboxylic group. The haptens were conjugated with bovine serum albumin (BSA) and rabbits were immunized with the conjugates. The obtained polyclonal antibodies showed good reactivity and the LOD of the constructed ELISAs was in the range 0.006-0.254 ng mL. Thus, they are suitable for the development of immunochemical assay kits.
色胺类是一类致幻药物，通过免疫化学检测试剂盒可简化其在体液中的检测。这些检测所用的抗体是通过用与目标分析物相似的半抗原与合适蛋白质的缀合物免疫实验动物获得的。因此，我们合成了基于色胺类药物的新型半抗原，选择了α，β-二甲基色胺（DMT）、5-甲氧基-α，β-二甲基色胺（5-MeO-DMT）和α，β-二异丙基色胺（DiPT）作为目标分析物。它们的结构用末端带有羧基的短连接子进行了修饰。将半抗原与牛血清白蛋白（BSA）缀合，并用缀合物免疫兔子。获得的多克隆抗体表现出良好的反应性，所构建的酶联免疫吸附测定（ELISA）的检测限在0.006 - 0.254 ng/mL范围内。因此，它们适用于免疫化学检测试剂盒的开发。
