Meehan C J, Davidson P M, Young D G, Foulis A K
Pancreas. 1987;2(1):91-8. doi: 10.1097/00006676-198701000-00014.
An animal model in which part of the pancreas was made diabetic due to almost total loss of insulin-secreting B cells, while the remainder of the gland remained normal, is described. In rabbits, a vascular clamp was placed across the junction of the body and tail of the pancreas, thus occluding the circulation to the tail. Alloxan (200 mg/kg) was injected i.v., and 4 min later dextrose (0.5 g/kg) was given by the same route. After a further 2 min the clamp was removed. Thirty-four animals were studied, 17 of which died in the first postoperative week of surgical complications or of alloxan-induced toxicity to the liver and kidneys. The survivors were killed between 4 and 12 weeks after surgery and were not metabolically diabetic. They had a virtually complete absence of B cells but a normal population of A, D, and PP cells in the head and body of the pancreas. The islets in the tail of the pancreas appeared entirely normal. This model appears suitable for studying the effects of locally produced insulin on pancreatic exocrine function in metabolically normal animals.
本文描述了一种动物模型，其中胰腺的一部分因胰岛素分泌β细胞几乎完全丧失而出现糖尿病，而腺体的其余部分保持正常。在兔子中，将血管夹置于胰腺体部和尾部的交界处，从而阻断尾部的血液循环。静脉注射四氧嘧啶（200mg/kg），4分钟后通过相同途径给予葡萄糖（0.5g/kg）。再过2分钟后移除血管夹。对34只动物进行了研究，其中17只在术后第一周死于手术并发症或四氧嘧啶对肝脏和肾脏的毒性作用。存活的动物在手术后4至12周被处死，且没有代谢性糖尿病。它们的β细胞几乎完全缺失，但胰腺头部和体部的A、D和PP细胞数量正常。胰腺尾部的胰岛看起来完全正常。该模型似乎适合研究局部产生的胰岛素对代谢正常动物胰腺外分泌功能的影响。
