Zhang Xiaobin, Dai Chengli, You Yuanyuan, He Lizhen, Chen Tianfeng
The First Affiliated Hospital, Department of Chemistry, Jinan University Guangzhou 510632 China
RSC Adv. 2018 Mar 21;8(21):11305-11315. doi: 10.1039/c8ra00151k.
A comprehensive assessment was conducted in this study to examine the antioxidant and antitumor activities of tea extract produced by Tie Guanyin hybridization. Two radical-scavenging systems of assay , namely ABTS and DPPH assays, were used to investigate the antioxidant activity of the summer tea and autumn tea extract (STE and ATE) derived from the Jin Guanyin. The results indicated that the major active ingredients were catechins, and the theaflavin is rare in the STE and ATE. Moreover, STE and ATE could significantly suppress the growth of human breast cancer cells MDA-MB-231 in a dose-dependent manner, and wrecked the morphology of mitochondria, activated caspase families, leading to the cancer cell death by both apoptosis and cell cycle arrest pathways. Based on the results from an MDA-MB-231 xenograft nude mice model, STE could effectively prevent the tumor formation, and greatly improve the mice immunity and thus improve their living conditions. Taken together, ATE and STE could act as a healthy and prospective substitute for natural antioxidants and a promising prophylactic agent against cancer. This finding provides a great promising nutritional approach to treat diseases related with oxidative stress.
本研究进行了全面评估，以考察铁观音杂交产生的茶叶提取物的抗氧化和抗肿瘤活性。采用两种自由基清除测定系统，即ABTS和DPPH测定法，来研究源自金观音的夏茶和秋茶提取物（STE和ATE）的抗氧化活性。结果表明，主要活性成分是儿茶素，而茶黄素在STE和ATE中含量稀少。此外，STE和ATE能以剂量依赖的方式显著抑制人乳腺癌细胞MDA-MB-231的生长，破坏线粒体形态，激活半胱天冬酶家族，通过凋亡和细胞周期阻滞途径导致癌细胞死亡。基于MDA-MB-231异种移植裸鼠模型的结果，STE能有效预防肿瘤形成，并极大地提高小鼠免疫力，从而改善其生存状况。综上所述，ATE和STE可作为天然抗氧化剂的健康且有前景的替代品，以及一种有前途的癌症预防剂。这一发现为治疗与氧化应激相关的疾病提供了一种极有前景的营养方法。
