Yun Jianrong, Duan Fang, Liu Liming, Chen Xiulai, Liu Jia, Luo Qiuling, Wu Jing
School of Pharmaceutical Sciences, Jiangnan University 1800 Lihu Road Wuxi 214122 Jiangsu Province P. R. China
School of Chemical and Material Engineering, Jiangnan University Wuxi 214122 Jiangsu Province P. R. China.
RSC Adv. 2018 Jan 17;8(7):3547-3555. doi: 10.1039/c7ra10377h. eCollection 2018 Jan 16.
Food allergies are increasingly recognized as a major healthcare concern. In order to sensitively and specifically detect allergies from blood samples of at-risk allergic patients, an effective magnetic fluorescence sensing platform (EMFP) was constructed. The EMFP incorporated hollow mesoporous silica nanospheres (HMNs) to amplify signal from the target IgE in addition to magnetic nanoparticles (MNPs) to capture and separate the target IgE. The application of EMFP to immunoassays indicated a detection limit of 0.0159 ng mL for low concentration specific immunoglobulin E (sIgE) against purified shellfish (Meta. E.) allergens, which is 15 fold more sensitive than the commercially available Food and Drug Administration-approved analyzers. Notably, EMFP was specific for the targeted sIgE even with interference by other sIgEs. In addition, the detection time is only 75 min, considerably faster than current commercial ELISA kits for IgE assays. Together, these results demonstrated that EMFP has excellent sensitivity and selectivity for the rapid detection of sIgE. The method thus exhibits potential toward the rapid monitoring of sIgE against Meta. E. allergens in clinical application.
食物过敏日益被视为一个重大的医疗保健问题。为了灵敏且特异地从高危过敏患者的血液样本中检测过敏反应，构建了一种有效的磁荧光传感平台（EMFP）。除了用于捕获和分离目标免疫球蛋白E（IgE）的磁性纳米颗粒（MNP）外，EMFP还纳入了中空介孔二氧化硅纳米球（HMN）以放大来自目标IgE的信号。EMFP在免疫分析中的应用表明，针对纯化的贝类（Meta. E.）过敏原的低浓度特异性免疫球蛋白E（sIgE）的检测限为0.0159 ng/mL，比美国食品药品监督管理局批准的市售分析仪灵敏15倍。值得注意的是，即使受到其他sIgE的干扰，EMFP对目标sIgE仍具有特异性。此外，检测时间仅为75分钟，比目前用于IgE检测的商用ELISA试剂盒快得多。总之，这些结果表明EMFP对sIgE的快速检测具有出色的灵敏度和选择性。因此，该方法在临床应用中对针对Meta. E.过敏原的sIgE的快速监测具有潜力。
