Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Hierarchical Green-Energy Materials (Hi-GEM) Research Center, National Cheng Kung University, Tainan, Taiwan.
FEBS Lett. 2022 Jul;596(14):1827-1838. doi: 10.1002/1873-3468.14375. Epub 2022 May 18.
Lipid-like ion-pair amphiphile vesicles, or catanionic vesicles, have emerged as potential drug carriers. The effects of cholesterol on the properties of catanionic vesicles have yet been systematically studied. Here, we compared the effects of cholesterol on the structures and fluidities of dipalmitoylphosphatidylcholine liposomes and catanionic vesicles with similar main transition temperatures (T ). For liposomes, fluorescence anisotropy (FA) thermograms reveal typical condensing and disordering effects of cholesterol above and below T respectively. In contrast, FA and molecular simulation data reveal that catanionic bilayers below T are more fluidic due to shorter alkyl chains. This leads to only condensing effects of cholesterol for catanionic vesicles at all temperatures. Our results provide important insights into the fabrication of catanionic vesicles as novel drug carriers.
类脂离子对两亲体囊泡，或双阳离子囊泡，已成为有潜力的药物载体。胆固醇对双阳离子囊泡性质的影响尚未得到系统研究。在这里，我们比较了胆固醇对具有相似主相变温度 (T) 的二棕榈酰磷脂酰胆碱脂质体和双阳离子囊泡结构和流动性的影响。对于脂质体，荧光各向异性 (FA) 热图谱分别揭示了胆固醇在 T 以上和以下的典型凝聚和去有序效应。相比之下，FA 和分子模拟数据表明，由于烷基链较短，T 以下的双阳离子双层更具流动性。这导致胆固醇对所有温度下的双阳离子囊泡仅具有凝聚效应。我们的结果为作为新型药物载体的双阳离子囊泡的制备提供了重要的见解。
