Homma Yuto, Sugawara Akihiro, Morishita Yohei, Tsukada Kento, Ozaki Taro, Asai Teigo
Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Org Lett. 2022 May 20;24(19):3504-3509. doi: 10.1021/acs.orglett.2c01172. Epub 2022 May 11.
Genome mining and bioinformatics analyses allowed us to rationally find a candidate biosynthetic gene cluster for a new cyclic depsipeptide of . A heterologous reconstitution of the identified biosynthetic pathway predictably afforded a new cyclic depsipeptide composed of l-leucine, l-tryptophan, and a polyketide moiety. Interestingly, the 10-membered macrocycle structure generated equilibrium to an unprecedented cyclol structure. This study demonstrates the advantage of a synthetic biology method in achieving rational access to new natural products.
基因组挖掘和生物信息学分析使我们能够合理地找到一种新型环缩肽的候选生物合成基因簇。对鉴定出的生物合成途径进行异源重建，不出所料地得到了一种由L-亮氨酸、L-色氨酸和一个聚酮部分组成的新型环缩肽。有趣的是，这个十元大环结构与一种前所未有的环醇结构形成了平衡。这项研究证明了合成生物学方法在合理获取新天然产物方面的优势。
