Zhang Jiangong, Fan Fei, Yu Tao, Chen Jianpei, Wan Minxi, Li Yuanguang
State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Biotechnol Lett. 2022 Jun;44(5-6):767-775. doi: 10.1007/s10529-022-03256-9. Epub 2022 May 11.
To investigate the actually received light of cells in the photo bioreactor, a light attenuation model of Synechocystis sp. PCC 6803 was established. The relationship between the average number of photons received per biomass (APRPB) and the growth of cell was analyzed. The results demonstrated, Cornet model was accurately fitted with the light attenuation of Synechocystis sp. PCC 6803 and the cell growth rate was affected by APRPB. When the value of APRPB is 3.2 μmol g s, the cell have the maximum light efficiency. A maximum specific growth rate of 0.05 h was achieved with APRPB from 3.2 to 12.8 μmol g s. After 156 h cultivation, compared to cells cultured under constant light [light intensity: 100 and 1800 μmol/(m s)], the DCW under controlled light intensity (light intensity increasing with the cell density) was higher by 79.1% and 20.0%, respectively. This study indicated that APRPB could be used as a light intensity regulation criterion to improve cell production despite different types of reactor and cell density, which provided a theoretical basis for improving the biomass yield of Synechocystis sp. PCC 6803 or other photosynthetic auto-trophic organism.
为了研究光生物反应器中细胞实际接收的光，建立了聚球藻属PCC 6803的光衰减模型。分析了每生物量接收的光子平均数（APRPB）与细胞生长之间的关系。结果表明，Cornet模型能准确拟合聚球藻属PCC 6803的光衰减，且细胞生长速率受APRPB的影响。当APRPB的值为3.2 μmol g s时，细胞具有最大光效率。APRPB在3.2至12.8 μmol g s范围内时，最大比生长速率达到0.05 h。培养156小时后，与在恒定光照下（光强：100和1800 μmol/(m² s)）培养的细胞相比，在光强随细胞密度增加的控制光照下的干重分别高出79.1%和20.0%。本研究表明，尽管反应器类型和细胞密度不同，APRPB仍可作为光强调节标准来提高细胞产量，这为提高聚球藻属PCC 6803或其他光合自养生物的生物量产量提供了理论依据。
