土耳其伊斯坦布尔一家三级医院的丙型肝炎病毒基因型和病毒血症。

Hepatitis C virus genotypes and viremia in a tertiary hospital in Istanbul, Turkey.

机构信息

University of Health Sciences, Istanbul Training and Research Hospital, Department of Microbiology, Istanbul, Turkey.

University of Health Sciences, Gulhane Training and Research Hospital, Department of Microbiology, Ankara, Turkey.

出版信息

J Infect Dev Ctries. 2022 Apr 30;16(4):668-674. doi: 10.3855/jidc.15256.

DOI:10.3855/jidc.15256

PMID:35544629

Abstract

INTRODUCTION

The World Health Organization estimates that 71 million people with chronic HCV infection lived worldwide in 2015. HCV is a globally prevalent pathogen, that genotype1 is the most common. In this study, the prevalence of anti-HCV, distributions of HCV genotype, and viremia rates in patients with chronic hepatitis C were evaluated.

METHODOLOGY

In this retrospective single-center study, anti-HCV results of 197,081 patients were evaluated between 2017 and 2020. Quantitative HCV-RNA PCR tests were performed on the Rotor-Gene Q real-time PCR instrument. HCV genotypes determination of 546 samples was carried out with the Gen-C 2.0 Reverse Hybridization strip and HCV Genotype Plus Real-TM kit.

RESULTS

The prevalence of anti-HCV was 0.95% and viremic HCV infection was 0.3% (610/197,081). HCV viremia rate was 33.17%. HCV viremia rate was highest in 2017 (52.36%) and the lowest in 2020 (18.3%) (p < 0.001). Genotype1 (72%) was the most common genotype, followed by genotype3 (14.1%), and genotype4 (8.8%). The most common subtypes were determined as genotype1b (56.2%) and genotype1a (13.2%). The viral load was higher in patients infected with genotype5.

CONCLUSIONS

In this study, the rate of viremic HCV infection was found to be 0.3%. This rate was lower than the worldwide rate of HCV viremia. The distribution of HCV genotypes was like the global data. The identification of circulating genotypes and subtypes is essential for epidemiological purposes and remains important in the choice of treatment in patients with chronic HCV.

摘要

简介

世界卫生组织估计，2015 年全球有 7100 万慢性 HCV 感染者。HCV 是一种全球流行的病原体，基因型 1 是最常见的。本研究评估了慢性丙型肝炎患者抗 HCV 的流行率、HCV 基因型分布和病毒血症率。

方法

在这项回顾性单中心研究中，评估了 2017 年至 2020 年期间 197081 例患者的抗 HCV 结果。使用 Rotor-Gene Q 实时 PCR 仪器对定量 HCV-RNA PCR 进行检测。使用 Gen-C 2.0 Reverse Hybridization 条带和 HCV Genotype Plus Real-TM 试剂盒对 546 例样本进行 HCV 基因型测定。

结果

抗 HCV 的流行率为 0.95%，病毒血症 HCV 感染率为 0.3%（197081 例中有 610 例）。HCV 病毒血症率为 33.17%。HCV 病毒血症率在 2017 年最高（52.36%），在 2020 年最低（18.3%）（p<0.001）。基因型 1（72%）是最常见的基因型，其次是基因型 3（14.1%）和基因型 4（8.8%）。最常见的亚型确定为基因型 1b（56.2%）和基因型 1a（13.2%）。感染基因型 5 的患者的病毒载量较高。