a:1:{s:5:"en_US";s:37:"Tehran University of Medical sciences";}.
1- Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran .
Acta Biomed. 2022 May 11;93(2):e2022027. doi: 10.23750/abm.v93i2.10823.
Shigella is a main cause of gastroenteritis and it is responsible for 5 to 10 % of diarrhea through the world. The aims of this study were to assess the antibiotic susceptibility pattern and the presence of 3 common virulence genes (sigA, virF, invE) of Shigella strains isolated from patients with gastroenteritis in Children's Medical Center Hospital, Tehran, Iran.
Over a period of 15 months, all Shigella species collected from the patients with gastroenteritis were entered to the study. Susceptibility testing of all isolates towards different antibiotics was performed using the disk diffusion method and the prevalence of virulence genes was detected by polymerase chain reaction (PCR) technology.
Among a total of 183 Shigella strains, 128 Shigella sonnei (70%) and 55 S. flexneri (30%) were isolated. The resistance rate to the antibiotics in S. sonnei strains was higher than S. flexneri. The most sensitive antibiotics for S. flexneri strains were gentamicin (98%), amikacin (85%) and ciprofloxacin (82%), while high resistance rate to trimethoprim-sulfamethoxazole (96%), ampicillin (96%), nalidixic acid (64%) and cefotaxime (60%) was observed. The frequency of invE, virF and sigA gene in S. flexneri strains was 89 %, 93 % and 56 %, respectively; whereas they found in 93 %, 96 %, and 100 % of S. sonnei strains, respectively. SigA gene was identified significantly higher in the S. sonnei strains (100%). There was no significant difference between the presence of virF and invE genes among Shigella strains.
The high presence of sigA gene in S. sonnei strains plays an important role in its pathogenesis, and the high frequency of invE and virF genes showed that this classical pathway regulating the expression of Shigella virulence factor genes could play a key role in the pathogenesis of this bacterium.
志贺氏菌是一种主要的肠胃炎病原体，它在全球范围内导致 5%至 10%的腹泻。本研究旨在评估从伊朗德黑兰儿童医学中心肠胃炎患者中分离的志贺氏菌菌株的抗生素敏感性模式和 3 种常见毒力基因（sigA、virF、invE）的存在情况。
在 15 个月的时间内，将所有从肠胃炎患者中分离的志贺氏菌种都纳入了本研究。使用纸片扩散法对所有分离株进行抗生素敏感性试验，采用聚合酶链反应（PCR）技术检测毒力基因的流行情况。
在总共 183 株志贺氏菌中，128 株为宋内志贺氏菌（70%），55 株为福氏志贺氏菌（30%）。宋内志贺氏菌对抗生素的耐药率高于福氏志贺氏菌。福氏志贺氏菌对庆大霉素（98%）、阿米卡星（85%）和环丙沙星（82%）最敏感，而对甲氧苄啶-磺胺甲恶唑（96%）、氨苄西林（96%）、萘啶酸（64%）和头孢噻肟（60%）的耐药率较高。福氏志贺氏菌中 invE、virF 和 sigA 基因的频率分别为 89%、93%和 56%，而宋内志贺氏菌中分别为 93%、96%和 100%。宋内志贺氏菌 sigA 基因的检出率明显更高（100%）。志贺氏菌中 virF 和 invE 基因的存在无显著差异。
宋内志贺氏菌中 sigA 基因的高存在与其发病机制有关，invE 和 virF 基因的高频出现表明，这种调节志贺氏菌毒力因子基因表达的经典途径可能在该细菌的发病机制中发挥关键作用。
