采用尿滤纸样本进行甲基丙二酸分析，以筛查老年人群代谢性维生素 B 缺乏症。

Methylmalonic acid analysis using urine filter paper samples to screen for metabolic vitamin B deficiency in older adults.

机构信息

Division of Medical Genetics, Department of Pediatrics, Centre de Recherche - Centre Hospitalier Universitaire de Sherbrooke, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada.

Research Center on Aging, Centre Intégré Universitaire de Santé et de Services Sociaux de l'Estrie - Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC, J1H 4C4, Canada.

出版信息

Bioanalysis. 2022 May;14(9):615-626. doi: 10.4155/bio-2022-0007. Epub 2022 May 12.

DOI:10.4155/bio-2022-0007

PMID:35546317

Abstract

Methylmalonic acid (MMA) analysis in urine represents a noninvasive approach to screening for vitamin B deficiency in older adults. A method allowing the analysis of MMA/creatinine in fasting urine collected on filter paper was developed/validated. Dry urine specimens were eluted using a solution containing internal standards, filtrated and analyzed by ultra-performance LC-MS/MS. The method allowed the chromatographic separation of MMA from succinic acid. Dried urine samples were stable for 86 days at room temperature. The MMA/creatinine ratios measured in urine collected on filter paper were highly correlated with values derived from the corresponding liquid specimens. This robust filter paper method might greatly improve the accessibility and cost-effectiveness of vitamin B deficiency screening in older adults.

摘要

尿液中甲基丙二酸（MMA）的分析可作为一种非侵入性方法，用于筛查老年人的维生素 B 缺乏症。本研究开发/验证了一种可分析滤纸采集的空腹尿液中 MMA/肌酐的方法。使用含有内标的溶液洗脱干燥的尿样，过滤后用超高效 LC-MS/MS 进行分析。该方法可实现 MMA 与琥珀酸的色谱分离。室温下，干燥的尿样在 86 天内稳定。滤纸采集的尿样中 MMA/肌酐比值与相应的液相标本所得值高度相关。这种稳健的滤纸法可能会极大地提高老年人维生素 B 缺乏症筛查的可及性和成本效益。