Faculty of Basic Sciences, Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Arch Razi Inst. 2021 Dec 30;76(6):1655-1663. doi: 10.22092/ari.2020.351381.1522. eCollection 2021 Dec.
Methicillin-resistant (MRSA) can cause infections in both human and animal groups, which is a serious threat to public health worldwide. Attachment and colonization are the first steps for pathogenesis, and biofilm-mediated infections have a significant negative impact on human and animal health. The MRSA can adapt to different environments and give rise to different strains of human and animal MRSA, causing transmissions of the disease between humans and animals. This study aimed to investigate biofilm production in vitro, and the presence of genes in MRSA isolates in both human as well as the disease transmission between human and animal strains. In total, 39 human and 35 livestock isolates were evaluated by the Congo Red Agar method. The presence of and genes were assessed by polymerase chain reaction (PCR), and finally, the PCR products were examined by agarose gel electrophoresis. The results showed that the gene frequency in human and animal isolates was 64.1% and 36.1%, respectively, and there was a significant relationship between and in human isolates. In addition, significant relationships were found between and Rifampicin and also between and Chloramphenicol and Penicillin in human isolates. In animal isolates, there was a significant relationship between mecA and Trimethoprim as well as between and Rifampicin. It was concluded that all operon genes were involved in biofilm production, but and genes in MRSA were more closely associated with . Both animal and human strains can be involved in disease transmission, but this conclusion should be made cautiously.
耐甲氧西林金黄色葡萄球菌（MRSA）可引起人类和动物群体的感染，这对全球公共卫生构成了严重威胁。附着和定植是发病机制的第一步，生物膜介导的感染对人类和动物健康有重大负面影响。MRSA 可以适应不同的环境，并产生不同的人类和动物 MRSA 菌株，导致疾病在人类和动物之间传播。本研究旨在调查体外生物膜的产生，以及人类和动物 MRSA 分离株中 基因的存在，以及人类和动物菌株之间的疾病传播。总共评估了 39 个人类和 35 个牲畜分离株，采用刚果红琼脂法。通过聚合酶链反应（PCR）评估 基因和 基因的存在，最后通过琼脂糖凝胶电泳检查 PCR 产物。结果表明，人类和动物分离株中 基因的频率分别为 64.1%和 36.1%，并且人类分离株中 基因和 基因之间存在显著关系。此外，在人类分离株中还发现了 基因和 Rifampicin 之间以及 基因和 Chloramphenicol 和 Penicillin 之间存在显著关系。在动物分离株中，mecA 基因和 Trimethoprim 基因以及 基因和 Rifampicin 基因之间存在显著关系。研究结论认为，所有操纵子 基因都参与了生物膜的产生，但 MRSA 中的 基因和 基因与 基因的关系更为密切。动物和人类菌株都可能参与疾病传播，但应谨慎得出这一结论。
