Omidi Mitra, Firoozeh Farzaneh, Saffari Mahmood, Sedaghat Hossein, Zibaei Mohammad, Khaledi Azad
Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
BMC Res Notes. 2020 Jan 7;13(1):19. doi: 10.1186/s13104-020-4885-9.
This study aimed to evaluate the phenotypic and genotypic characterization of biofilm formation and spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus.
This cross-sectional study was performed on 146 Staphylococcus aureus isolates from hospitalized patients in Isfahan Province Hospitals. MRSA isolates were confirmed using disk diffusion test with oxacillin disk and amplification of mecA gene by PCR assays. Ability of biofilm production was evaluated targeting the icaA and icaD genes. Of 146 Staphylococcus aureus isolates, 24 (16.4%) carried mecA genes and identified as MRSA strains. Strong ability of biofilm production was seen among 76.02% (111/146) S. aureus isolates and 87.5% (21/24) MRSA strains, respectively. Also, 75.0% (18/24) MRSA isolates carried icaA and icaD was not detected in these strains. Analysis of spa gene showed 70.83% (17/24) MRSA strains were spa positive. From which 14 and 3 strains identified with one band (150, 270, 300, 360, 400 bp) and two bands (150-300 bp), respectively. According to data obtained, the prevalence of MRSA isolates from Isfahan Province Hospitals is relatively high and a remarkable percentage of them show strong power in biofilm production. Also analysis of spa gene showed a fairly large diversity among MRSA strains.
本研究旨在评估耐甲氧西林金黄色葡萄球菌临床分离株中生物膜形成以及spa和ica基因的表型和基因型特征。
本横断面研究对来自伊斯法罕省医院住院患者的146株金黄色葡萄球菌分离株进行了检测。采用苯唑西林纸片扩散试验及PCR法扩增mecA基因来确认耐甲氧西林金黄色葡萄球菌（MRSA）分离株。通过靶向icaA和icaD基因评估生物膜产生能力。在146株金黄色葡萄球菌分离株中，24株（16.4%）携带mecA基因，被鉴定为MRSA菌株。金黄色葡萄球菌分离株和MRSA菌株中分别有76.02%（111/146）和87.5%（21/24）表现出较强的生物膜产生能力。此外，75.0%（18/24）的MRSA分离株携带icaA基因，且在这些菌株中未检测到icaD基因。spa基因分析显示70.83%（17/24）的MRSA菌株为spa阳性。其中，分别有14株和3株菌株鉴定为一条带（150、270、300、360、400 bp）和两条带（150 - 300 bp）。根据获得的数据，伊斯法罕省医院MRSA分离株的患病率相对较高，其中相当一部分表现出较强的生物膜产生能力。spa基因分析还显示MRSA菌株之间存在相当大的多样性。
