Xu Xiaoyan, Zhang Yi, Wang Bingfeng, Luo Lin, Xu Zhenlin, Tian Xingguo
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University Guangzhou 510642 PR China
Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University Guangzhou 510642 PR China.
RSC Adv. 2018 Sep 19;8(57):32538-32544. doi: 10.1039/c8ra06295a. eCollection 2018 Sep 18.
Herein, a novel surface plasmon resonance (SPR) sensor based on a functionalized graphene oxide (GO)/molecular-imprinted polymer composite was developed for the chiral recognition of l-tryptophan (l-Trp). The composite's recognition element was prepared a facile and green synthesis approach using polydopamine as both a reducer of GO and a functional monomer as well as a cross-linker for molecular imprinting. The composite was characterized Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and Raman spectroscopy. After attaching the composite onto the gold surface of an SPR chip, the sensor was characterized using contact-angle measurements. The sensor exhibited excellent selectivity and chiral recognition for the template (, l-Trp). Density functional theory computations showed that the difference in hydrogen bonding between the composite element and l-Trp and d-Trp played an important role in chiral recognition.
在此，开发了一种基于功能化氧化石墨烯（GO）/分子印迹聚合物复合材料的新型表面等离子体共振（SPR）传感器，用于对L-色氨酸（L-Trp）进行手性识别。该复合材料的识别元件采用一种简便且绿色的合成方法制备，使用聚多巴胺作为GO的还原剂、功能单体以及分子印迹的交联剂。通过傅里叶变换红外光谱、扫描电子显微镜、X射线衍射和拉曼光谱对该复合材料进行了表征。将该复合材料附着到SPR芯片的金表面后，使用接触角测量对传感器进行了表征。该传感器对模板（即L-Trp）表现出优异的选择性和手性识别能力。密度泛函理论计算表明，复合材料元件与L-Trp和D-Trp之间氢键的差异在手性识别中起重要作用。
