Fulton Chandler
Department of Biology, Brandeis University, Waltham, MA, United States.
Front Cell Dev Biol. 2022 Apr 25;10:867374. doi: 10.3389/fcell.2022.867374. eCollection 2022.
The multi-tubulin hypothesis proposed in 1976 was motivated by finding that the tubulin to build the flagellar apparatus was synthesized during the optional differentiation of from walking amoebae to swimming flagellates. In the next decade, with the tools of cloning and sequencing, we were able to establish that the rate of flagellar tubulin synthesis in is determined by the abundance of flagellar - and -tubulin mRNAs. These experiments also established that the tubulins for mitosis were encoded by separate, divergent genes, candidates for which remain incompletely characterized. Meanwhile an unanticipated abundance of tubulin isotypes has been discovered by other researchers. Together with the surprises of genome complexity, these tubulin isotypes require us to rethink how we might utilize the opportunities and challenges offered by the evolutionary diversity of eukaryotes.
1976年提出的多微管蛋白假说是基于这样一个发现：构建鞭毛器的微管蛋白是在变形虫从爬行向有鞭毛的游动形态转变的选择性分化过程中合成的。在接下来的十年里，借助克隆和测序工具，我们得以确定，[此处原文缺失相关生物名称]中鞭毛微管蛋白的合成速率由鞭毛α-和β-微管蛋白mRNA的丰度决定。这些实验还证实，用于[此处原文缺失相关生物名称]有丝分裂的微管蛋白由不同的、分化的基因编码，而这些基因的候选基因仍未得到充分表征。与此同时，其他研究人员发现了数量惊人的微管蛋白异构体。连同基因组复杂性带来的意外情况，这些微管蛋白异构体要求我们重新思考如何利用真核生物进化多样性带来的机遇和挑战。
