高效液相色谱法药物分析方法发展的若干选定方面
Selected aspects of the development of methods for the analysis of drugs by high performance liquid chromatography.
作者信息
Riley C M
出版信息
Xenobiotica. 1987 Mar;17(3):365-83. doi: 10.3109/00498258709043945.
The successful analysis of drugs in biological fluids and other matrices by reversed phase high performance liquid chromatography (h.p.l.c.) relies upon the optimization of the chromatographic separation, the sample preparation and the post-column detection. Recent advances in the detection and derivatisation of exogenous substances are described elsewhere in this volume (Sternson 1986). This paper deals with selected aspects of the optimization of the chromatographic separation, concentrating on the theoretical basis and practical application of mobile phase selectivity in reversed phase ion-pair systems. In addition, recent advances in sample preparation techniques which permit the direct injection of biological fluids are discussed.
通过反相高效液相色谱法（h.p.l.c.）成功分析生物流体和其他基质中的药物，依赖于色谱分离、样品制备和柱后检测的优化。本卷其他地方描述了外源物质检测和衍生化的最新进展（斯特恩森，1986年）。本文讨论了色谱分离优化的选定方面，重点是反相离子对系统中流动相选择性的理论基础和实际应用。此外，还讨论了允许直接进样生物流体的样品制备技术的最新进展。
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