Tang Wei, Cheng Mengxi, Dai Danling, Xiong Zhonghua, Liu Feng
Institute of Materials, China Academy of Engineering Physics Mianyang 621700 China
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University Beijing 100871 China.
RSC Adv. 2018 Aug 17;8(51):29338-29343. doi: 10.1039/c8ra05757e. eCollection 2018 Aug 14.
DNAzymes as functional units play increasingly important roles for DNA nanotechnology, and fine control of the catalytic activities of DNAzymes is a crucial element in the design and construction of functional and dynamic devices. So far, attempts to control cleavage kinetics can be mainly achieved through varying the concentrations of the specific metal ions. Here we present a facile sequestered DNAzyme beacon strategy based on precisely blocking the catalytic core of the DNAzyme, which can flexibly regulate the DNAzyme cleavage kinetics without changing the concentrations of metal ions. This strategy can be extended to couple with a large number of other RNA-cleaving DNAzymes and was successfully applied in designing a dual stem-loop structure probe for arbitrary sequence biosensing, which provides the possibility of scaling up versatile and dynamic DNA devices that use DNAzymes as functional modules.
脱氧核酶作为功能单元在DNA纳米技术中发挥着越来越重要的作用，而对脱氧核酶催化活性的精确控制是功能动态装置设计与构建的关键要素。到目前为止，控制切割动力学的尝试主要是通过改变特定金属离子的浓度来实现的。在此，我们提出了一种基于精确阻断脱氧核酶催化核心的简便的隔离脱氧核酶信标策略，该策略可以在不改变金属离子浓度的情况下灵活调节脱氧核酶的切割动力学。这种策略可以扩展到与大量其他切割RNA的脱氧核酶相结合，并成功应用于设计用于任意序列生物传感的双茎环结构探针，这为扩大使用脱氧核酶作为功能模块的通用动态DNA装置提供了可能性。
