Xu You D, Lai Rebecca Y, Procházková Eliška, Stenzel Martina H
Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney 2052, NSW, Australia.
Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Prague 166 10, Czech Republic.
ACS Macro Lett. 2021 Jul 20;10(7):819-824. doi: 10.1021/acsmacrolett.1c00270. Epub 2021 Jun 15.
Albumin has consistently demonstrated its potential for enhancing the delivery of drugs and polymer-drug conjugates, binding via supramolecular forces within its multiple binding sites. Herein, we introduce saturation transfer difference (STD-NMR) as a method to identify the interactions between a polymer library and bovine serum albumin (BSA). With STD-NMR, the binding ability of polymers can be quickly screened by focusing on their individual structural features, making this technique more suitable for high throughput screening in comparison to traditional fluorescence studies.
白蛋白一直显示出其增强药物和聚合物-药物偶联物递送的潜力，它通过其多个结合位点内的超分子力进行结合。在此，我们引入饱和转移差异（STD-NMR）作为一种鉴定聚合物文库与牛血清白蛋白（BSA）之间相互作用的方法。通过STD-NMR，可以通过关注聚合物的个体结构特征快速筛选其结合能力，与传统荧光研究相比，该技术更适合高通量筛选。
