Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Arch Oral Biol. 2022 Aug;140:105448. doi: 10.1016/j.archoralbio.2022.105448. Epub 2022 Apr 29.
This study aims to explore and compare the histopathology of upper cleft lip tissue in order to identify the abnormalities and orientation of muscle and collagen fibers in patients affected with non-syndromic cleft lip with or without cleft palate (NSCL±P) and normal controls.
Eight fresh lip tissues of consented patients with NSCL±P and two controls were fixed and stained with hematoxylin and eosin (H&E), Masson's trichrome (MT), and modified Gomori trichrome techniques. The images were captured and examined using imaging cellSens software (Olympus, Tokyo, Japan) and Mirax Scan (Carl Zeiss, Germany). The H&E stained tissues were analysed for muscle fiber size using image processing program (imageJ, USA). Histopathological appearance of epidermal and dermal layers including collagen orientation, as well as muscle fibers abnormalities were observed.
Tissues stained with H&E exhibit pseudoepitheliomatous hyperplasia, epidermal and sebaceous glands hyperplasia. Morphometric analysis of muscle fibers showed the diameter was between 6.912 and 10.246 µm. Collagen fibers were densely packed in cleft tissue, but muscle fibers were sparse in MT stain. Modified Gomori trichrome stain revealed hypoplastic muscle with fibrosis, including ragged red fibers.
Disoriented collagen fibers, significant sparse and disorganized orbicularis oris muscle fibers with classical myopathic appearances proved that cleft tissue had abnormal histology findings. These findings further support the mechanism of collagen and muscle fibers during embryonic development that causing cleft formation.
本研究旨在探索和比较上唇裂隙组织的组织病理学，以识别非综合征性唇裂伴或不伴腭裂（NSCL±P）患者和正常对照者的肌肉和胶原纤维的异常和取向。
8 例同意参与研究的 NSCL±P 患者和 2 例对照者的新鲜唇组织经固定和苏木精-伊红（H&E）、马松三色（MT）和改良 Gomori 三色技术染色。使用成像细胞 Sens 软件（Olympus，东京，日本）和 Mirax Scan（Carl Zeiss，德国）拍摄和检查图像。使用图像处理程序（imageJ，美国）分析 H&E 染色组织中的肌纤维大小。观察表皮和真皮层的表皮和真皮层的组织病理学表现，包括胶原纤维的取向和肌肉纤维的异常。
H&E 染色的组织显示假上皮瘤样增生、表皮和皮脂腺增生。肌纤维的形态计量分析显示直径在 6.912 至 10.246µm 之间。MT 染色显示裂隙组织中胶原纤维密集，但肌肉纤维稀疏。改良 Gomori 三色染色显示肌肉纤维发育不良伴纤维化，包括锯齿状红纤维。
紊乱的胶原纤维、稀疏和无序的口轮匝肌纤维，具有典型的肌病表现，证明裂隙组织有异常的组织病理学发现。这些发现进一步支持了胚胎发育过程中胶原和肌肉纤维的机制，导致了裂隙的形成。
