School of Chemical Engineering, Zhengzhou University, Zhengzhou, 450001, Henan, China; School of Civil Engineering, The University of Queensland, 4072, QLD, Australia.
School of Chemical Engineering, The University of Queensland, 4072, QLD, Australia.
J Environ Manage. 2022 Aug 15;316:115216. doi: 10.1016/j.jenvman.2022.115216. Epub 2022 May 9.
In treating mine-impacted waters using sulfate-reducing bacteria (SRB), metal inhibition and substrate selection are important factors affecting the efficiency of the bioprocess. This work investigated the role of the substrate (i.e. lactate, formate, glycerol and glucose) on Ni inhibition to SRB with sulfate-reducing activity tests at initial pH 5, 7 and 9 and 100 mg/L of Ni. Results indicated that the type of substrate was a significant factor affecting Ni inhibition in SRB, which was the most negligible in the lactate system, followed by glycerol, glucose, and formate. Although less significant, Ni inhibition also varied with the pH, leading for instance, to a reduction of 77% in the sulfate reducing activity for the formate system, but only of 28% for lactate at pH 5. The added substrate also influenced the precipitation kinetics and the characteristics of the precipitates, reaching Ni precipitation extents above 95%, except for glucose (83.2%).
在使用硫酸盐还原菌（SRB）处理矿山污染水时，金属抑制和底物选择是影响生物过程效率的重要因素。本研究在初始 pH 值为 5、7 和 9 以及 Ni 浓度为 100mg/L 的条件下，通过硫酸盐还原活性测试，考察了底物（即乳酸盐、甲酸盐、甘油和葡萄糖）对具有硫酸盐还原活性的 SRB 中 Ni 抑制的作用。结果表明，底物类型是影响 SRB 中 Ni 抑制的一个重要因素，在乳酸盐体系中，Ni 抑制作用最小，其次是甘油、葡萄糖和甲酸盐。尽管影响较小，但 Ni 抑制作用也随 pH 值而变化，例如，在 pH 值为 5 时，甲酸盐体系的硫酸盐还原活性降低了 77%，而乳酸盐体系仅降低了 28%。添加的底物也会影响沉淀动力学和沉淀的特性，除葡萄糖（83.2%）外，Ni 的沉淀率均超过 95%。
