Veldhuis J D, Guardabasso V, Rogol A D, Evans W S, Oerter K E, Johnson M L, Rodbard D
Am J Physiol. 1987 May;252(5 Pt 1):E599-605. doi: 10.1152/ajpendo.1987.252.5.E599.
We have appraised the nature of spontaneous luteinizing hormone (LH) secretory events in normal men by analyzing immunoactive LH concentrations in blood samples withdrawn at 5-min intervals for 24 h in eight healthy individuals. A novel discrete deconvolution algorithm was applied to determine apparent instantaneous LH secretory rates from these LH concentration series. These analyses unmasked unique attributes of spontaneous LH secretory events, which were represented as delimited momentary augmentations in endogenous LH secretory rates interspersed among intervals of relative secretory quiescence. For three different peak-detection thresholds (P = 0.02, = 0.01, and = 0.005), the frequency of LH secretory episodes was significantly greater than that of LH "pulses" judged from concentration data alone. Moreover, the total mass of LH released within discrete secretory episodes could account for approximately 90% of LH secreted during the day. This model of distinct, short-lived, burst-like secretion of LH has important implications for further investigations of the neuroendocrine regulation of gonadotropin secretion in humans.
我们通过分析8名健康个体在24小时内每隔5分钟采集的血样中免疫活性促黄体生成素（LH）的浓度，评估了正常男性自发性LH分泌事件的性质。应用一种新颖的离散反卷积算法从这些LH浓度序列中确定表观瞬时LH分泌率。这些分析揭示了自发性LH分泌事件的独特特征，其表现为内源性LH分泌率的限定性瞬时增加，穿插于相对分泌静止的间隔之中。对于三种不同的峰值检测阈值（P = 0.02、= 0.01和 = 0.005），LH分泌发作的频率显著高于仅根据浓度数据判断的LH“脉冲”频率。此外，离散分泌发作期间释放的LH总量约占白天分泌的LH的90%。这种LH独特、短暂、爆发样分泌的模型对进一步研究人类促性腺激素分泌的神经内分泌调节具有重要意义。
