Wang Xin-Sen, Hu Ming-Xin, Guan Qing-Xiang, Men Li-Hui, Liu Zhong-Ying
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Chin J Nat Med. 2022 May;20(5):378-386. doi: 10.1016/S1875-5364(22)60175-4.
The dry root and rhizome of Panax ginseng C. A. Mey has garnered much interest owing to its medicinal properties against diabetes and cardiovascular diseases. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS)-based metabolomics approach was used to illustrate the therapeutic mechanisms of ginseng extract on the serum and urinary metabolic profiles in streptozotocin-induced type 1 diabetes mellitus (T1DM) rats. Pharmacological and renal parameters in response to the administration of ginseng were also evaluated. In total, 16 serum endogenous metabolites and 14 urine endogenous metabolites, including pyruvic acid, indoleacetic acid, and phenylacetylglycine, were identified as potential biomarkers for diabetes. Pathway enrichment and network analysis revealed that the biomarkers modulated by ginseng were primarily involved in phenylalanine and pyruvate metabolism, as well as in arginine biosynthesis. Moreover, the levels of several renal injury-related biomarkers in T1DM rats were significantly restored following treatment with ginseng. The administration of the extract helped maintain tissue structure integrity and ameliorated renal injury. The findings suggest that the regulatory effect of ginseng extract on T1DM involves metabolic management of diabetic rats, which subsequently attenuates T1DM-induced early renal dysfunction.
人参（Panax ginseng C. A. Mey）的干燥根及根茎因其对糖尿病和心血管疾病的药用特性而备受关注。在本研究中，基于超高效液相色谱-四极杆飞行时间质谱联用技术（UHPLC-Q-TOF-MS）的代谢组学方法被用于阐明人参提取物对链脲佐菌素诱导的1型糖尿病（T1DM）大鼠血清和尿液代谢谱的治疗机制。同时也评估了给予人参后大鼠的药理学和肾脏参数。总共鉴定出16种血清内源性代谢物和14种尿液内源性代谢物，包括丙酮酸、吲哚乙酸和苯乙酰甘氨酸，作为糖尿病的潜在生物标志物。通路富集和网络分析表明，人参调节的生物标志物主要参与苯丙氨酸和丙酮酸代谢以及精氨酸生物合成。此外，T1DM大鼠经人参治疗后，几种与肾损伤相关的生物标志物水平显著恢复。提取物的给药有助于维持组织结构完整性并改善肾损伤。研究结果表明，人参提取物对T1DM的调节作用涉及对糖尿病大鼠的代谢管理，进而减轻T1DM诱导的早期肾功能障碍。
