生物炭和堆肥修复重金属污染土壤中碱性磷酸酶活性及 phoD 基因群落特征。
The characteristics of alkaline phosphatase activity and phoD gene community in heavy-metal contaminated soil remediated by biochar and compost.
机构信息
College of Resources and Environment, Hunan Agricultural University, 410128, Changsha, China.
School of Hydraulic Engineering, Engineering and Technical Center of Hunan Provincial Environmental Protection for River-Lake Dredging Pollution Control, Changsha University of Science & Technology, 410114, Changsha, China.
出版信息
Bull Environ Contam Toxicol. 2022 Aug;109(2):298-303. doi: 10.1007/s00128-022-03513-7. Epub 2022 May 13.
This research was carried out to determine the influence of biochar and compost addition on the characteristics of potential alkaline phosphatase (ALP) activity and phoD gene community in heavy metal polluted soils. The ALP activity, the abundance and structure of phoD gene were systematically determined. Results showed that biochar and compost significantly changed soil properties, and promoted the microbial transformation of phosphorus. Compost addition significantly increased the ALP activity. Biochar and compost addition markedly increased the phoD gene abundance. The addition of biochar increased the proportion of Actinobacteria, Euryarchaeota, and Proteobacteria. By contrast, Betaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria were the dominant taxa in soils with compost addition. Electrical conductivity critically controlled the expression of phoD and changed the structure of phoD-coding microbial communities in heavy-metal polluted soils that remediated by biochar and compost.
本研究旨在确定生物炭和堆肥添加对重金属污染土壤中潜在碱性磷酸酶（ALP）活性和 phoD 基因群落特征的影响。系统测定了 ALP 活性、phoD 基因丰度和结构。结果表明，生物炭和堆肥显著改变了土壤性质，促进了磷的微生物转化。添加堆肥显著提高了 ALP 活性。生物炭和堆肥添加显著增加了 phoD 基因丰度。生物炭的添加增加了放线菌门、广古菌门和变形菌门的比例。相比之下，添加堆肥的土壤中，β变形菌门、δ变形菌门和γ变形菌门是主要类群。电导率是控制 phoD 表达的关键因素，并改变了生物炭和堆肥修复的重金属污染土壤中 phoD 编码微生物群落的结构。
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