Grinde B, Hungnes O, Gravningen M
Biomed Biochim Acta. 1986;45(11-12):1627-33.
Isolated rat hepatocytes were treated either by electroshock or enterotoxin in order to perforate the plasma membrane. Under appropriate conditions perforated cells could be incubated for a couple of hours without further disruption. The rate of proteolysis was higher in the perforated cells than in homogenized cells. ATP, a thiol reagent and Ca2+/Mg2+ stimulated proteolysis. By using inhibitors, it was shown that the proteolysis was partly lysosomal, and that all four classes of endoproteinases were involved.
分离出的大鼠肝细胞用电击或肠毒素处理，以使质膜穿孔。在适当条件下，穿孔细胞可以孵育几个小时而不会进一步破裂。穿孔细胞中的蛋白水解速率高于匀浆细胞。ATP、一种硫醇试剂和Ca2+/Mg2+可刺激蛋白水解。通过使用抑制剂表明，蛋白水解部分是溶酶体性的，并且所有四类内肽酶都参与其中。
