Fuller G M, Bunzel R J, Woloski B M, Nham S U
Biochem Biophys Res Commun. 1987 Apr 29;144(2):1003-9. doi: 10.1016/s0006-291x(87)80063-3.
Hepatocyte stimulating factor is a monocyte derived protein which regulates hepatic plasma protein synthesis. Human hepatocyte stimulating factor was purified to apparent homogeneity from adherent peripheral blood monocytes by using ion exchange, gel permeation and reversed phase chromatography. Electrophoretic analysis showed that it has a Mr of 28,000 daltons and an isoelectric point of 5.4. Biological assays specific for hepatocyte stimulating factor and interleukin-1 showed that they are distinct and have independent biological effects.
肝细胞刺激因子是一种单核细胞衍生蛋白，可调节肝脏血浆蛋白的合成。通过离子交换、凝胶渗透和反相色谱法，从贴壁外周血单核细胞中纯化出了人肝细胞刺激因子，使其达到表观均一性。电泳分析表明，它的分子量为28,000道尔顿，等电点为5.4。针对肝细胞刺激因子和白细胞介素-1的生物学测定表明，它们是不同的，具有独立的生物学效应。
