Chekhonadskikh T V, Poliakova N E, Pankova T G, Salganik R I
Biokhimiia. 1987 Mar;52(3):381-6.
A microsomal fraction from the cells of the malaria parasite of rodent Plasmodium berghei was obtained. The spectral properties of microsomal preparations suggest that P. berghei microsomes contain cytochromes b5 and P-420. Electrophoretic separation of microsomal proteins revealed the presence of proteins whose molecular mass corresponds to NADPH-cytochrome c reductase, cytochrome P-450 and epoxide hydratase. The activities of NADPH-cytochrome c reductase and benzpyrene hydroxylase were determined. The spectral parameters, electrophoretic data and enzymatic activities of microsomal proteins indicate that P. berghei cells contain a cytochrome P-450 monooxygenase system. The interrelationship between the activity of the microsomal monooxygenase system and the resistance of P. berghei cells to the antimalaria preparation chloroquine is discussed.
从啮齿动物伯氏疟原虫的疟原虫细胞中获得了微粒体部分。微粒体制剂的光谱特性表明，伯氏疟原虫微粒体含有细胞色素b5和P-420。微粒体蛋白的电泳分离显示存在分子量与NADPH-细胞色素c还原酶、细胞色素P-450和环氧化物水合酶相对应的蛋白质。测定了NADPH-细胞色素c还原酶和苯并芘羟化酶的活性。微粒体蛋白的光谱参数、电泳数据和酶活性表明，伯氏疟原虫细胞含有细胞色素P-450单加氧酶系统。讨论了微粒体单加氧酶系统的活性与伯氏疟原虫细胞对抗疟制剂氯喹的抗性之间的相互关系。
