国际食品微生物标准委员会方法研究。十七。食品中大肠杆菌计数的直接平板法和疏水网格膜过滤法的国际比较研究。国际食品微生物标准委员会。

ICMSF methods study. XVII. An international comparative study of the direct plate and hydrophobic grid-membrane filter methods for enumeration of Escherichia coli in foods. International Commission on Microbiological Specifications for Foods.

作者信息

Sharpe A N, Rayman M K, Malik N, Beckers H J, Delfgou E, Christian J H, Eyles M, Dodsworth P, Nafziger M, Gibbs P A

出版信息

Can J Microbiol. 1987 Feb;33(2):85-92. doi: 10.1139/m87-015.

Abstract

Eight laboratories compared counts of Escherichia coli from naturally or artificially contaminated ground beef, other meats and poultry, vegetables, fish and shellfish, cheese, and diverse sources such as swabs, by the Anderson-Baird-Parker direct plate (DP) and a hydrophobic grid-membrane filter (HGMF) method. For five of the eight laboratories overall counts by HGMF were significantly low (51-83%) compared with those by DP. Counts by HGMF tended to be lower for naturally contaminated samples; several possible causes were investigated. In a subsidiary study, analyst variation in counting HGMF ranged from 0.8-7.3%, with little evidence of effects from counting positive versus negative grid cells or from the fullness of growth or staining intensity.

摘要

八个实验室采用安德森-贝尔德-帕克直接平板法（DP）和疏水网格膜过滤法（HGMF），对来自天然或人工污染的碎牛肉、其他肉类和家禽、蔬菜、鱼类和贝类、奶酪以及拭子等多种来源的大肠杆菌进行计数比较。在这八个实验室中，有五个实验室采用HGMF法得出的总计数与DP法相比显著偏低（51%-83%）。天然污染样本采用HGMF法得出的计数往往更低；对几种可能的原因进行了调查。在一项辅助研究中，分析人员对HGMF计数的差异范围为0.8%-7.3%，几乎没有证据表明对阳性与阴性网格细胞计数、生长饱满程度或染色强度有影响。