Wu Huina, Zou Mingyuan, Fan Xiaobo, Su Feiya, Xiao Feng, Zhou Meiling, Sun Yan, Zhao Fengfeng, Wu Guoqiu
Medical School of Southeast University, Nanjing 210009, People's Republic of China.
Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, People's Republic of China.
ACS Omega. 2022 Apr 19;7(17):15074-15081. doi: 10.1021/acsomega.2c00882. eCollection 2022 May 3.
Influenza viruses and respiratory syncytial virus (RSV) have contributed to severe respiratory infections, causing huge economic and healthcare burdens. To achieve rapid and precise detection of influenza viruses and RSV, we proposed a catalytic hairpin assembly (CHA) combined with the lateral flow immunoassay (CHA-LFIA) detection method. The presence of the target RNA triggers the initiation of CHA circuits. H1/H2 complexes, the amplified signal products, which were labeled with digoxin and biotin, were detected with a highly sensitive lateral flow immunoassay system. The sensitivity of the CHA-LFIA system to influenza A and B viruses and RSV reached up to 1, 1, and 5 pM, respectively. In addition, this method exhibited excellent capability for differentiating between target RNA and base-mismatched RNA. The results demonstrated that an enzyme-free, rapid, highly sensitive, and specific method had been developed to detect influenza A and B viruses and RSV.
流感病毒和呼吸道合胞病毒（RSV）可导致严重的呼吸道感染，造成巨大的经济和医疗负担。为了实现对流感病毒和RSV的快速、精确检测，我们提出了一种将催化发夹组装（CHA）与侧向流动免疫分析（CHA-LFIA）相结合的检测方法。目标RNA的存在会触发CHA回路的启动。用高灵敏度的侧向流动免疫分析系统检测用洋地黄毒苷和生物素标记的H1/H2复合物（扩增信号产物）。CHA-LFIA系统对甲型和乙型流感病毒以及RSV的灵敏度分别高达1、1和5 pM。此外，该方法在区分目标RNA和碱基错配RNA方面表现出优异的能力。结果表明，已开发出一种无酶、快速、高灵敏度且特异的方法来检测甲型和乙型流感病毒以及RSV。
