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基于催化DNA组装电路的流感病毒和呼吸道合胞病毒的简便、快速且低成本检测

Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit.

作者信息

Wu Huina, Zou Mingyuan, Fan Xiaobo, Su Feiya, Xiao Feng, Zhou Meiling, Sun Yan, Zhao Fengfeng, Wu Guoqiu

机构信息

Medical School of Southeast University, Nanjing 210009, People's Republic of China.

Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, People's Republic of China.

出版信息

ACS Omega. 2022 Apr 19;7(17):15074-15081. doi: 10.1021/acsomega.2c00882. eCollection 2022 May 3.

DOI:10.1021/acsomega.2c00882
PMID:35557683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9089383/
Abstract

Influenza viruses and respiratory syncytial virus (RSV) have contributed to severe respiratory infections, causing huge economic and healthcare burdens. To achieve rapid and precise detection of influenza viruses and RSV, we proposed a catalytic hairpin assembly (CHA) combined with the lateral flow immunoassay (CHA-LFIA) detection method. The presence of the target RNA triggers the initiation of CHA circuits. H1/H2 complexes, the amplified signal products, which were labeled with digoxin and biotin, were detected with a highly sensitive lateral flow immunoassay system. The sensitivity of the CHA-LFIA system to influenza A and B viruses and RSV reached up to 1, 1, and 5 pM, respectively. In addition, this method exhibited excellent capability for differentiating between target RNA and base-mismatched RNA. The results demonstrated that an enzyme-free, rapid, highly sensitive, and specific method had been developed to detect influenza A and B viruses and RSV.

摘要

流感病毒和呼吸道合胞病毒（RSV）可导致严重的呼吸道感染，造成巨大的经济和医疗负担。为了实现对流感病毒和RSV的快速、精确检测，我们提出了一种将催化发夹组装（CHA）与侧向流动免疫分析（CHA-LFIA）相结合的检测方法。目标RNA的存在会触发CHA回路的启动。用高灵敏度的侧向流动免疫分析系统检测用洋地黄毒苷和生物素标记的H1/H2复合物（扩增信号产物）。CHA-LFIA系统对甲型和乙型流感病毒以及RSV的灵敏度分别高达1、1和5 pM。此外，该方法在区分目标RNA和碱基错配RNA方面表现出优异的能力。结果表明，已开发出一种无酶、快速、高灵敏度且特异的方法来检测甲型和乙型流感病毒以及RSV。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/645f0b6d488f/ao2c00882_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/7486cd482870/ao2c00882_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/599edeb22736/ao2c00882_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/d5a929d329b1/ao2c00882_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/6d17260ca3a8/ao2c00882_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/645f0b6d488f/ao2c00882_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/7486cd482870/ao2c00882_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/599edeb22736/ao2c00882_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/d5a929d329b1/ao2c00882_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/6d17260ca3a8/ao2c00882_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff0e/9089383/645f0b6d488f/ao2c00882_0005.jpg

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Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit.

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引用本文的文献

[1]
Detection of by Catalytic Hairpin Assembly Combined with a Lateral Flow Immunoassay Strip.

ACS Omega. 2022-9-15

本文引用的文献

[1]
Excess respiratory mortality and hospitalizations associated with influenza in Australia, 2007-2015.

Int J Epidemiol. 2022-5-9

[2]
A fluorescent microarray platform based on catalytic hairpin assembly for MicroRNAs detection.

Anal Chim Acta. 2021-8-15

[3]
One-step fast and label-free imaging array for multiplexed detection of trace avian influenza viruses.

Anal Chim Acta. 2021-8-1

[4]
SPR/SERS dual-mode plasmonic biosensor via catalytic hairpin assembly-induced AuNP network.

Biosens Bioelectron. 2021-10-15

[5]
Rapid point-of-care testing for SARS-CoV-2 virus nucleic acid detection by an isothermal and nonenzymatic Signal amplification system coupled with a lateral flow immunoassay strip.

Sens Actuators B Chem. 2021-9-1

[6]
AS03-adjuvanted H7N9 inactivated split virion vaccines induce cross-reactive and protective responses in ferrets.

NPJ Vaccines. 2021-3-19

[7]
Development of integrated microfluidic platform coupled with Surface-enhanced Raman Spectroscopy for diagnosis of COVID-19.

Med Hypotheses. 2021-1

[8]
Pulmonary function analysis in cotton rats after respiratory syncytial virus infection.

PLoS One. 2020-8-10

[9]
Prospects and Challenges in the Development of Universal Influenza Vaccines.

Vaccines (Basel). 2020-7-6

[10]
Mesoscale All-Atom Influenza Virus Simulations Suggest New Substrate Binding Mechanism.

ACS Cent Sci. 2020-2-26

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