Division of Bio-Resource Sciences, Bio-Herb Research Institute, and Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, South Korea.
Front Cell Infect Microbiol. 2022 Apr 26;12:861915. doi: 10.3389/fcimb.2022.861915. eCollection 2022.
is the major anthracnose fungus of sweet pepper and chili pepper ( L.), causing significant losses in the yield and quality of the pepper fruits. Molecular mechanisms governing development and pathogenicity have been widely studied in many foliar fungal pathogens, but the information on fruit diseases is still limited. In this study, we determined the functional roles of the dual-specificity tyrosine phosphorylation-regulated kinase CsPOM1 in . Knockout mutant for gene was obtained homology-dependent gene replacement. The mutant exhibited a reduction in vegetative growth on osmotic stress, surface hydrophobicity, and conidiation compared with wild-type. Conidia of the mutant were already two-celled before inoculation on an induction surface, indicating that negatively regulates conidial cell division. The mutant, similar to wild-type, formed appressoria on the plant surface, but was significantly reduced on hydrophobic coverslips, probably due to a defect in the recognition of surface hydrophobicity. Treatment of conidia with cutin monomers restored appressorium formation on hydrophobic coverslips in the mutant. On pepper fruits, the mutant exhibited delayed penetration and invasive growth, leading to significantly reduced virulence. Collectively, the results showed that is important for stress tolerance, conidiation, surface hydrophobicity, appressorium formation, and virulence in .
是甜椒和辣椒（L.）的主要炭疽病真菌，导致辣椒果实的产量和质量显著下降。许多叶部真菌病原体的发育和致病性的分子机制已经得到了广泛研究，但关于果实病害的信息仍然有限。在这项研究中，我们确定了双特异性酪氨酸磷酸化调节激酶 CsPOM1 在 中的功能作用。通过同源依赖性基因替换获得了基因的敲除突变体。与野生型相比，基因敲除突变体在渗透胁迫、表面疏水性和分生孢子形成方面的营养生长减少。与野生型相比，突变体的分生孢子在接种诱导表面之前已经是两细胞，表明 负调控分生孢子细胞分裂。突变体与野生型相似，在植物表面形成附着胞，但在疏水载玻片上的附着胞数量明显减少，可能是由于对表面疏水性的识别缺陷所致。用角质单体处理分生孢子可恢复突变体在疏水载玻片上的附着胞形成。在辣椒果实上，突变体表现出穿透和侵袭生长的延迟，导致毒力显著降低。总的来说，结果表明在 中， 对于应激耐受、分生孢子形成、表面疏水性、附着胞形成和毒力都是很重要的。
