Yekke-Ghasemi Zahra, Takjoo Reza, Ramezani Mohammad, Mague Joel T
Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad Mashhad Iran
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences Mashhad Iran.
RSC Adv. 2018 Dec 14;8(73):41795-41809. doi: 10.1039/c8ra07100d. eCollection 2018 Dec 12.
The syntheses of a new set of metal complexes MoOL'(CHOH), VOL'(CHO)(CHOH), , , SnL'Cl and SnL'I with a new ligand (L = (2,2'(disulfanediylbis((ethylthio)methylene)bis(hydrazin-2-yl-1-ylidene)bis(methanylylidene)) diphenol; L' = -ethyl-3-(2-hydroxyphenyl)methylenedithiocarbazate are described along with characterization by elemental analysis, mass spectrometry, spectroscopic (IR, H- and C-NMR) and TGA techniques. The crystal structures of compounds were determined by single crystal X-ray diffraction analysis and compared to powder X-ray diffraction (PXRD) patterns of the nano complexes obtained using ultrasonic methods. The PXRD results indicate that the compounds synthesized by ultrasonic methods have high crystallinity. The compounds were evaluated in an cytotoxicity study with two human cancer cell lines. The results of this study revealed that all complexes exhibit good cytotoxic activity when compared to the clinical drug, cisplatin. Interaction of the samples with human serum albumin (HSA) was investigated using fluorescence spectrophotometric methods and the Stern-Volmer quenching constant ( ) and free energy changes (Δ) were calculated at 298 K. The fluorescence quenching method is used to determine the number of binding sites () and association constants ( ) at the same temperatures.
描述了一组新的金属配合物MoOL'(CHOH)、VOL'(CHO)(CHOH)、SnL'Cl和SnL'I的合成，其中使用了一种新配体（L = (2,2'(二硫代二亚基双((乙硫基)亚甲基)双(肼-2-基-1-亚基)双(亚甲基))二酚；L' = -乙基-3-(2-羟基苯基)亚甲基二硫代氨基甲酸盐），并通过元素分析、质谱、光谱（红外、氢和碳核磁共振）和热重分析技术进行了表征。通过单晶X射线衍射分析确定了化合物的晶体结构，并与使用超声方法获得的纳米配合物的粉末X射线衍射（PXRD）图谱进行了比较。PXRD结果表明，通过超声方法合成的化合物具有高结晶度。在对两种人类癌细胞系的细胞毒性研究中对这些化合物进行了评估。该研究结果表明，与临床药物顺铂相比，所有配合物均表现出良好的细胞毒性活性。使用荧光分光光度法研究了样品与人血清白蛋白（HSA）的相互作用，并在298 K下计算了斯特恩-沃尔默猝灭常数（）和自由能变化（Δ）。在相同温度下，使用荧光猝灭法确定结合位点数量（）和缔合常数（）。
